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Literature DB >> 3841128

Effect of polybrene on isolation of Chlamydia trachomatis from clinical specimens.

Abstract

Polybrene treatment of McCoy cells was used to increase the infectivity of Chlamydia trachomatis in vitro. The isolation rate for C. trachomatis in 500 specimens from males and females attending a sexually transmitted disease clinic and other clinics increased by 6.8% with the Polybrene treatment. The data also suggested that this treatment facilitated the diagnosis of low grade or latent infections.

Entities: Chemical Species

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Year: 1985 PMID： 3841128 PMCID： PMC268493 DOI： 10.1128/jcm.22.4.671-673.1985

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

15 in total

1. Centrifugation and Rickettsiae and viruses onto cells and its effect on infection.

Authors: E WEISS; H R DRESSLER
Journal: Proc Soc Exp Biol Med Date: 1960-04

2. Comparison of various McCoy cell treatment procedures used for detection of Chlamydia trachomatis.

Authors: R T Evans; D Taylor-Robinson
Journal: J Clin Microbiol Date: 1979-08 Impact factor: 5.948

Review 3. The chlamydia: molecular biology of procaryotic obligate parasites of eucaryocytes.

Authors: Y Becker
Journal: Microbiol Rev Date: 1978-06

4. Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions.

Authors: K Toyoshima; P K Vogt
Journal: Virology Date: 1969-07 Impact factor: 3.616

5. Infection of cell cultures by trachoma agent: enhancement by DEAE-dextran.

Authors: T R Rota; R L Nichols
Journal: J Infect Dis Date: 1971-10 Impact factor: 5.226

6. Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran.

Authors: C Kuo; S Wang; B B Wentworth; J T Grayston
Journal: J Infect Dis Date: 1972-06 Impact factor: 5.226

7. Effect of polycations, polyanions and neuraminidase on the infectivity of trachoma-inclusin conjunctivitis and lymphogranuloma venereum organisms HeLa cells: sialic acid residues as possible receptors for trachoma-inclusion conjunction.

Authors: C C Kuo; S P Wang; J T Grayston
Journal: Infect Immun Date: 1973-07 Impact factor: 3.441

8. Efficacy of various cell culture procedures for detection of Chlamydia trachomatis and applicability to diagnosis of pediatric infections.

Authors: L J La Scolea; J E Keddell
Journal: J Clin Microbiol Date: 1981-04 Impact factor: 5.948

Effect of polybrene on isolation of Chlamydia trachomatis from clinical specimens.

1. Centrifugation and Rickettsiae and viruses onto cells and its effect on infection.

2. Comparison of various McCoy cell treatment procedures used for detection of Chlamydia trachomatis.

Review 3. The chlamydia: molecular biology of procaryotic obligate parasites of eucaryocytes.

4. Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions.

5. Infection of cell cultures by trachoma agent: enhancement by DEAE-dextran.

6. Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran.

7. Effect of polycations, polyanions and neuraminidase on the infectivity of trachoma-inclusin conjunctivitis and lymphogranuloma venereum organisms HeLa cells: sialic acid residues as possible receptors for trachoma-inclusion conjunction.

8. Efficacy of various cell culture procedures for detection of Chlamydia trachomatis and applicability to diagnosis of pediatric infections.

9. Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells.

10. Isolation of Chlamydia trachomatis by use of 5-iodo-2-deoxyuridine-treated cells.

1. In vitro tetracycline susceptibility of Chlamydia trachomatis in clinical specimens.

2. Factors affecting viability and growth in HeLa 229 cells of Chlamydia sp. strain TWAR.