Literature DB >> 3840509

Detection of eosinophils using an eosinophil peroxidase assay. Its use as an assay for eosinophil differentiation factors.

M Strath, D J Warren, C J Sanderson.   

Abstract

A colorimetric assay for peroxidase has been applied to the detection of eosinophils in bone marrow cultures and tissue cell suspensions. The substrate solution consists of 0.1 mM o-phenylenediamine in 0.05 M Tris-HCl buffer pH 8.0 containing 0.1% Triton and 1 mM hydrogen peroxide. The method is shown to be an easy and reproducible method of detecting eosinophils, with insignificant interference from neutrophils.

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Year:  1985        PMID： 3840509     DOI： 10.1016/0022-1759(85)90242-x

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  73 in total

1.  Role of airway eosinophilia and eosinophil activation in Sephadex-induced inflammation.

Authors:  Karim Maghni; François Nantel; Chantal Lanoue; Solange Cloutier; Jean-Paul Cristol; Alain Cadieux; Pierre Sirois
Journal:  Inflammation       Date:  2004-08       Impact factor: 4.092

2.  A small molecule, orally active, alpha4beta1/alpha4beta7 dual antagonist reduces leukocyte infiltration and airway hyper-responsiveness in an experimental model of allergic asthma in Brown Norway rats.

Authors:  Julio Cortijo; María-Jesús Sanz; Arantxa Iranzo; José Luis Montesinos; Yafa Naim Abu Nabah; José Alfón; Luis A Gómez; Manuel Merlos; Esteban J Morcillo
Journal:  Br J Pharmacol       Date:  2006-03       Impact factor: 8.739

3.  The development of a sensitive and specific ELISA for mouse eosinophil peroxidase: assessment of eosinophil degranulation ex vivo and in models of human disease.

Authors:  Sergei I Ochkur; John Dongil Kim; Cheryl A Protheroe; Dana Colbert; Redwan Moqbel; Paige Lacy; James J Lee; Nancy A Lee
Journal:  J Immunol Methods       Date:  2011-10-12       Impact factor: 2.303

4.  Expression of interleukin-9 leads to Th2 cytokine-dominated responses and fatal enteropathy in mice with chronic Schistosoma mansoni infections.

Authors:  P G Fallon; P Smith; E J Richardson; F J Jones; H C Faulkner; J Van Snick; J C Renauld; R K Grencis; D W Dunne
Journal:  Infect Immun       Date:  2000-10       Impact factor: 3.441

5.  Aberrant inflammatory response to Streptococcus pyogenes in mice lacking myeloid differentiation factor 88.

Authors:  Torsten G Loof; Oliver Goldmann; André Gessner; Heiko Herwald; Eva Medina
Journal:  Am J Pathol       Date:  2009-12-17       Impact factor: 4.307

6.  Ablation of tumor progression locus 2 promotes a type 2 Th cell response in Ovalbumin-immunized mice.

Authors:  Wendy T Watford; Chun-Chi Wang; Christos Tsatsanis; Lisa A Mielke; Aristides G Eliopoulos; Constantine Daskalakis; Nicolas Charles; Sandra Odom; Juan Rivera; John O'Shea; Philip N Tsichlis
Journal:  J Immunol       Date:  2009-12-02       Impact factor: 5.422

7.  Trimellitic anhydride-induced allergic response in the lung: role of the complement system in cellular changes.

Authors:  D G Fraser; J F Regal; M L Arndt
Journal:  J Pharmacol Exp Ther       Date:  1995-05       Impact factor: 4.030

8.  The inhibition of antigen-induced eosinophilia and bronchoconstriction by CDP840, a novel stereo-selective inhibitor of phosphodiesterase type 4.

Authors:  B Hughes; D Howat; H Lisle; M Holbrook; T James; N Gozzard; K Blease; P Hughes; R Kingaby; G Warrellow; R Alexander; J Head; E Boyd; M Eaton; M Perry; M Wales; B Smith; R Owens; C Catterall; S Lumb; A Russell; R Allen; M Merriman; D Bloxham; G Higgs
Journal:  Br J Pharmacol       Date:  1996-07       Impact factor: 8.739

9.  Intranasal challenge with increasing ovalbumin doses differently affects airway hyperresponsiveness and inflammatory cell accumulation in mouse model of asthma.

Authors:  Berislav Bosnjak; Vanesa Ivetić Tkalcević; Koraljka Durić; Daniela Belamarić; Snjezana Cuzić; Zeljko Ferencić; Karmen Brajsa; Ines Glojnarić; Roberto Antolović; Boska Hrvacić
Journal:  Inflamm Res       Date:  2009-05-08       Impact factor: 4.575

10.  Schistosoma mansoni antigens modulate experimental allergic asthma in a murine model: a major role for CD4+ CD25+ Foxp3+ T cells independent of interleukin-10.

Authors:  Lucila G G Pacífico; Fábio A V Marinho; Cristina T Fonseca; Michele M Barsante; Vanessa Pinho; Policarpo A Sales-Junior; Luciana S Cardoso; Maria Ilma Araújo; Edgar M Carvalho; Geovanni D Cassali; Mauro M Teixeira; Sergio C Oliveira
Journal:  Infect Immun       Date:  2008-09-29       Impact factor: 3.441
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