Literature DB >> 3839755

N-terminal amino acid sequence and some properties of isopenicillin-N synthetase from Cephalosporium acremonium.

J E Baldwin, J Gagnon, H Ting.   

Abstract

Isopenicillin-N synthetase (IPNS) was purified to homogeneity from Cephalosporium acremonium C0728. The enzyme existed in two states during purification; an oxidised state with a disulphide linkage and its reduced state. These two forms can be interconverted in the presence or absence of thiol agents, and separated by fast protein liquid chromatography (FPLC) with the strong anion exchange Mono-Q column. The enzyme is a monomer with a molecular mass of 38 kDa and pI 5.05. The first 50 amino acid N-terminal sequence of the enzyme was determined. The purified enzyme has an absolute requirement of Fe2+ and a 2-electron donor for activity.

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Year:  1985        PMID： 3839755     DOI： 10.1016/0014-5793(85)80382-3

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  7 in total

1.  Purification to homogeneity and characterization of acyl coenzyme A:6-aminopenicillanic acid acyltransferase of Penicillium chrysogenum.

Authors:  E Alvarez; J M Cantoral; J L Barredo; B Díez; J F Martín
Journal:  Antimicrob Agents Chemother       Date:  1987-11       Impact factor: 5.191

2.  Photoaffinity labelling of isopenicillin N synthetase.

Authors:  J E Baldwin; J B Coates; M G Moloney; A J Pratt; A C Willis
Journal:  Biochem J       Date:  1990-03-01       Impact factor: 3.857

Review 3.  Molecular regulation of beta-lactam biosynthesis in filamentous fungi.

Authors:  A A Brakhage
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

4.  Active-site-mediated elimination of hydrogen fluoride from a fluorinated substrate analogue by isopenicillin N synthase.

Authors:  Annaleise R Grummitt; Peter J Rutledge; Ian J Clifton; Jack E Baldwin
Journal:  Biochem J       Date:  2004-09-01       Impact factor: 3.857

5.  Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities.

Authors:  J E Baldwin; R M Adlington; J B Coates; M J Crabbe; N P Crouch; J W Keeping; G C Knight; C J Schofield; H H Ting; C A Vallejo
Journal:  Biochem J       Date:  1987-08-01       Impact factor: 3.857

6.  Factors affecting the isopenicillin N synthetase reaction.

Authors:  D Perry; E P Abraham; J E Baldwin
Journal:  Biochem J       Date:  1988-10-01       Impact factor: 3.857

7.  Beta-lactam biosynthesis in a gram-negative eubacterium: purification and characterization of isopenicillin N synthase from Flavobacterium sp. strain SC 12.154.

Authors:  H Palissa; H von Döhren; H Kleinkauf; H H Ting; J E Baldwin
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490
  7 in total

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