Literature DB >> 3839457

Trypanosoma brucei: a surface antigen mRNA is discontinuously transcribed from two distinct chromosomes.

M Guyaux, A W Cornelissen, E Pays, M Steinert, P Borst.   

Abstract

The mRNAs for variant surface glycoproteins (VSGs) and many other proteins in Trypanosoma brucei start with the same sequence of 35 nucleotides, encoded by a separate mini-exon. There are approximately 200 mini-exon genes per trypanosome and these are highly clustered on large chromosomes. We have found two trypanosome variants that express a VSG gene located on a small, 225-kb chromosome. Each gene yields a mRNA containing the 35-nucleotide sequence even though the 225-kb chromosome does not contain a complete mini-exon gene. These results provide a strong support for the hypothesis that transcription of protein-coding genes in trypanosomes is discontinuous.

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Year:  1985        PMID： 3839457      PMCID： PMC554290          DOI： 10.1002/j.1460-2075.1985.tb03729.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  39 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Antigenic variation in syringe passaged populations of Trypanosoma (Trypanozoon) brucei. 1. Rationalization of the experimental approach.

Authors:  N Van Meirvenne; P G Janssens; E Magnus
Journal:  Ann Soc Belg Med Trop       Date:  1975

3.  Multiple arrangements of viral DNA and an activated host oncogene in bursal lymphomas.

Authors:  G S Payne; J M Bishop; H E Varmus
Journal:  Nature       Date:  1982-01-21       Impact factor: 49.962

4.  Gene duplication and transposition linked to antigenic variation in Trypanosoma brucei.

Authors:  E Pays; N Van Meirvenne; D Le Ray; M Steinert
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

5.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

6.  Evidence for diploidy and mating in trypanosomes.

Authors:  A Tait
Journal:  Nature       Date:  1980-10-09       Impact factor: 49.962

7.  Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose.

Authors:  S M Lanham; D G Godfrey
Journal:  Exp Parasitol       Date:  1970-12       Impact factor: 2.011

8.  A physical map of the DNA regions flanking the rabbit beta-globin gene.

Authors:  A J Jeffreys; R A Flavell
Journal:  Cell       Date:  1977-10       Impact factor: 41.582

9.  A novel method to map transcripts: evidence for homology between an adenovirus mRNA and discrete multiple regions of the viral genome.

Authors:  A R Dunn; J A Hassell
Journal:  Cell       Date:  1977-09       Impact factor: 41.582

10.  Novel expression-linked copies of the genes for variant surface antigens in trypanosomes.

Authors:  J H Hoeijmakers; A C Frasch; A Bernards; P Borst; G A Cross
Journal:  Nature       Date:  1980-03-06       Impact factor: 49.962
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  15 in total

Review 1.  mRNA processing in the Trypanosomatidae.

Authors:  K Perry; N Agabian
Journal:  Experientia       Date:  1991-02-15

2.  Characterization of VSG gene expression site promoters and promoter-associated DNA rearrangement events.

Authors:  K Gottesdiener; H M Chung; S D Brown; M G Lee; L H Van der Ploeg
Journal:  Mol Cell Biol       Date:  1991-05       Impact factor: 4.272

3.  Analysis of the transcript encoding the latent Epstein-Barr virus nuclear antigen I: a potentially polycistronic message generated by long-range splicing of several exons.

Authors:  S H Speck; J L Strominger
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

4.  A 5' exo-ribonuclease and RNA ligase of T. brucei.

Authors:  J Huang; L H Van der Ploeg
Journal:  Nucleic Acids Res       Date:  1988-10-25       Impact factor: 16.971

5.  Evidence for a class of very small introns in the gene for hypoxanthine-guanine phosphoribosyltransferase in Schistosoma mansoni.

Authors:  S P Craig; M G Muralidhar; J H McKerrow; C C Wang
Journal:  Nucleic Acids Res       Date:  1989-02-25       Impact factor: 16.971

6.  Trypanosoma brucei: enrichment by UV of intergenic transcripts from the variable surface glycoprotein gene expression site.

Authors:  H Coquelet; P Tebabi; A Pays; M Steinert; E Pays
Journal:  Mol Cell Biol       Date:  1989-09       Impact factor: 4.272

7.  Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei.

Authors:  S Alexandre; M Guyaux; N B Murphy; H Coquelet; A Pays; M Steinert; E Pays
Journal:  Mol Cell Biol       Date:  1988-06       Impact factor: 4.272

8.  Structure and sequence of the gene for the largest subunit of trypanosomal RNA polymerase III.

Authors:  J Köck; R Evers; A W Cornelissen
Journal:  Nucleic Acids Res       Date:  1988-09-26       Impact factor: 16.971

9.  Physical identification of branched intron side-products of splicing in Trypanosoma brucei.

Authors:  D Ralph; J Huang; L H Van der Ploeg
Journal:  EMBO J       Date:  1988-08       Impact factor: 11.598

10.  Structure and regulated expression of genes encoding fructose biphosphate aldolase in Trypanosoma brucei.

Authors:  C E Clayton
Journal:  EMBO J       Date:  1985-11       Impact factor: 11.598
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