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Literature DB >> 3838595

Insertion mutagenesis of embryonal carcinoma cells by retroviruses.

W King, M D Patel, L I Lobel, S P Goff, M C Nguyen-Huu.

Abstract

Mutagenesis was studied in cultured F9 embryonal carcinoma cells infected with a variant of Moloney murine leukemia virus. Proviral insertion induced the inactivation of the hypoxanthine phosphoribosyltransferase locus, and the virus was used to isolate the mutated genes rapidly. Mutagenesis by these methods may be useful for the genetic dissection of the various mammalian cell phenotypes.

Entities: Disease Species

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Year: 1985 PMID： 3838595 DOI： 10.1126/science.3838595

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

40 in total

1. HIV-1 promotor insertion revealed by selective detection of chimeric provirus-host gene transcripts.

Authors: I Raineri; H P Senn
Journal: Nucleic Acids Res Date: 1992-12-11 Impact factor: 16.971

2. Viral sequences are associated with many histocompatibility genes.

Authors: A Rossomando; D Meruelo
Journal: Immunogenetics Date: 1986 Impact factor: 2.846

3. Transcriptionally active genome regions are preferred targets for retrovirus integration.

Authors: U Scherdin; K Rhodes; M Breindl
Journal: J Virol Date: 1990-02 Impact factor: 5.103

4. Measurement of human immunodeficiency virus type 1 preintegration transcription by using Rev-dependent Rev-CEM cells reveals a sizable transcribing DNA population comparable to that from proviral templates.

Authors: Subashini R Iyer; Dongyang Yu; Angélique Biancotto; Leonid B Margolis; Yuntao Wu
Journal: J Virol Date: 2009-06-24 Impact factor: 5.103

5. Derivation of genetically modified human pluripotent stem cells with integrated transgenes at unique mapped genomic sites.

Authors: Eirini P Papapetrou; Michel Sadelain
Journal: Nat Protoc Date: 2011-08-04 Impact factor: 13.491

6. Isolation and characterization of a Chinese hamster ovary mutant cell line with altered sensitivity to vaccinia virus killing.

Authors: C H Bair; C S Chung; I A Vasilevskaya; W Chang
Journal: J Virol Date: 1996-07 Impact factor: 5.103

Insertion mutagenesis of embryonal carcinoma cells by retroviruses.

1. HIV-1 promotor insertion revealed by selective detection of chimeric provirus-host gene transcripts.

2. Viral sequences are associated with many histocompatibility genes.

3. Transcriptionally active genome regions are preferred targets for retrovirus integration.

4. Measurement of human immunodeficiency virus type 1 preintegration transcription by using Rev-dependent Rev-CEM cells reveals a sizable transcribing DNA population comparable to that from proviral templates.

5. Derivation of genetically modified human pluripotent stem cells with integrated transgenes at unique mapped genomic sites.

6. Isolation and characterization of a Chinese hamster ovary mutant cell line with altered sensitivity to vaccinia virus killing.

7. Effect of proviral insertion on transcription of the murine B2mb gene.

8. Identification of cellular promoters by using a retrovirus promoter trap.

9. Positive genetic selection for gene disruption in mammalian cells by homologous recombination.

Review 10. Provirus tagging as an instrument to identify oncogenes and to establish synergism between oncogenes.