Literature DB >> 3838336

Purification and characterization of the respiratory syncytial virus fusion protein.

E E Walsh, M W Brandriss, J J Schlesinger.   

Abstract

The fusion protein of respiratory syncytial virus was purified by affinity chromatography using a monoclonal antibody. Under various conditions the protein was recovered as a 145K dimer or a 70K monomer. The 70K monomer was composed of disulphide-linked fragments of 48K and 23K. Polyclonal rabbit serum produced to the dimerized fusion protein neutralized virus but did not inhibit fusion, while rabbit serum to the 2-mercaptoethanol-treated dimerized protein neutralized virus and inhibited fusion of infected cells. Only the latter serum strongly recognized the 23K fragment when studied by Western blot analysis.

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Year:  1985        PMID： 3838336     DOI： 10.1099/0022-1317-66-3-409

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  39 in total

1.  Antibody response to the central unglycosylated region of the respiratory syncytial virus attachment protein in mice.

Authors:  Yoshihiko Murata; Seana C Catherman
Journal:  Vaccine       Date:  2012-06-19       Impact factor: 3.641

2.  Monoclonal antibodies to the fusion protein of bovine respiratory syncytial virus.

Authors:  K M Mulkey; G A Anderson
Journal:  J Clin Microbiol       Date:  1991-09       Impact factor: 5.948

3.  Circulating antibody-secreting cells during acute respiratory syncytial virus infection in adults.

Authors:  F Eun-Hyung Lee; Ann R Falsey; Jessica L Halliley; Iñaki Sanz; Edward E Walsh
Journal:  J Infect Dis       Date:  2010-10-27       Impact factor: 5.226

4.  Comparison of caprine, human and bovine strains of respiratory syncytial virus.

Authors:  M Trudel; F Nadon; C Simard; F Bélanger; R Alain; C Séguin; G Lussier
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

5.  Antigenically important proteins of Aujeszky's disease (pseudorabies) virus identified by immunoblotting.

Authors:  D Todd; J Hull; J McNair
Journal:  Arch Virol       Date:  1987       Impact factor: 2.574

6.  Respiratory syncytial virus-specific antibody responses in immunoglobulin A and E isotypes to the F and G proteins and to intact virus after natural infection.

Authors:  R C Welliver; M Sun; S W Hildreth; R Arumugham; P L Ogra
Journal:  J Clin Microbiol       Date:  1989-02       Impact factor: 5.948

7.  Amino acid variation within the fusion protein of respiratory syncytial virus subtype A and B strains during annual epidemics in South Africa.

Authors:  Elizabeth Agenbach; Caroline T Tiemessen; Marietjie Venter
Journal:  Virus Genes       Date:  2005-03       Impact factor: 2.332

Review 8.  Hyperimmune globulins in prevention and treatment of respiratory syncytial virus infections.

Authors:  V G Hemming; G A Prince; J R Groothuis; G R Siber
Journal:  Clin Microbiol Rev       Date:  1995-01       Impact factor: 26.132

9.  Expression of the F glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the F and G glycoproteins to host immunity.

Authors:  R A Olmsted; N Elango; G A Prince; B R Murphy; P R Johnson; B Moss; R M Chanock; P L Collins
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

10.  Productive infection of isolated human alveolar macrophages by respiratory syncytial virus.

Authors:  J R Panuska; N M Cirino; F Midulla; J E Despot; E R McFadden; Y T Huang
Journal:  J Clin Invest       Date:  1990-07       Impact factor: 14.808
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