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Literature DB >> 383661

Consecutive demonstration of catecholamines and dopamine-beta-hydroxylase within the same specimen.

Abstract

A combined method was developed for characterization and differentiation of catecholamines in neuron populations containing more than one catecholamine, e.g. dopamine and norepinephrine. Its application to small intensely fluorescent (SIF-) cell clusters in sympathetic ganglia allows the successive demonstration of glyoxylic acid-induced catecholamine fluorescence and dopamine-beta-hydroxylase by indirect immunofluorescence within the same tissue section. Applying this technique to an example, two types of SIF-cells were demonstrated in the guinea pig superior cervical ganglion.

Entities: Chemical Gene Species

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Year: 1979 PMID： 383661 DOI： 10.1007/bf00508451

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

18 in total

1. A rapid, simple and sensitive method for the demonstration of central catecholamine-containing neurons and axons by glyoxylic acid-induced fluorescence. II. A detailed description of methodology.

Authors: F E Bloom; E L Battensberg
Journal: J Histochem Cytochem Date: 1976-04 Impact factor: 2.479

2. Histochemically demonstrable catecholamines and cholinesterases in nerve fibres of rat dorsal skin.

Authors: T Waris; L Rechardt
Journal: Histochemistry Date: 1977-08-22

3. SIF cells, cyclic AMP responses, and catecholamines of the guinea pig superior cervical ganglion.

Authors: J K Wamsley; A C Black; J A Redick; J R West; T H Williams
Journal: Brain Res Date: 1978-11-03 Impact factor: 3.252

4. Histochemical and biochemical characterization of the rat paracervical ganglion.

Authors: H A Baker; J P Burke; R K Bhatnagar; D E Van Order; L S Van Orden; B K Hartman
Journal: Brain Res Date: 1977-09-02 Impact factor: 3.252

5. Fluorescence histochemistry of biogenic monoamines. A study of the capacity of various carbonyl compounds to form fluorophores with biogenic monoamines in gas phase reactions.

Authors: S Axelsson; A Björklund; O Lindvall
Journal: J Histochem Cytochem Date: 1972-06 Impact factor: 2.479

Review 6. Immunofluorescence of dopamine- -hydroxylase. Application of improved methodology to the localization of the peripheral and central noradrenergic nervous system.

Authors: B K Hartman
Journal: J Histochem Cytochem Date: 1973-04 Impact factor: 2.479

7. Glyoxylic acid condensation: a new fluorescence method for the histochemical demonstration of biogenic monoamines.

Authors: S Axelsson; A Björklund; B Falck; O Lindvall; L A Svensson
Journal: Acta Physiol Scand Date: 1973-01

8. The use of glyoxylic acid for the fluorescence histochemical demonstration of peripheral stores of noradrenaline and 5-hydroxytryptamine in whole mounts.

Authors: J B Furness; M Costa
Journal: Histochemistry Date: 1975

9. Dopamine -hydroxylase of bovine adrenal medullae. A rapid purification procedure.

Authors: A Foldes; P L Jeffrey; B N Preston; L Austin
Journal: Biochem J Date: 1972-03 Impact factor: 3.857

10. A methodological approach to rapid and sensitive monoamine histofluorescence using a modified glyoxylic acid technique: the SPG method.

Authors: J C Torre; J W Surgeon
Journal: Histochemistry Date: 1976-10-22

3 in total