Literature DB >> 383073

Effect of fixation time on measurement of 2'-deoxyribonucleoside 5'-triphosphate pools in the rat embyro.

E J Ritter, W J Scott, L M Bruce.   

Abstract

Values for the concentrations of deoxyribonucleoside triphosphates in rat embryos on day 12 of gestation, determined by high-pressure liquid chromatography, were artifactually two to three times as high in embryos fixed by cooling in ice/water followed by freezing on solid CO2, in 20s, as in those more rapidly/fixed in liquid N2, in 1 s.

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Year:  1979        PMID： 383073      PMCID： PMC1186684          DOI： 10.1042/bj1790715

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  [A simple technic for extremely rapid freezing of large pieces of tissue].

Authors:  A WOLLENBERGER; O RISTAU; G SCHOFFA
Journal:  Pflugers Arch Gesamte Physiol Menschen Tiere       Date:  1960

2.  Regional levels of choline and acetylcholine in rat brain following head focussed microwave sacrifice: effect of (+)-amphetamine and (+/-)-parachloroamphetamine.

Authors:  D E Schmidt
Journal:  Neuropharmacology       Date:  1976-02       Impact factor: 5.250

3.  Free nucleotides of the brain in various mammals.

Authors:  P MANDEL; S HARTH
Journal:  J Neurochem       Date:  1961-11       Impact factor: 5.372

Review 4.  Analysis of nucleotide pools in animal cells.

Authors:  P V Hauschka
Journal:  Methods Cell Biol       Date:  1973       Impact factor: 1.441

5.  Factors determining cell sensitivity to methotrexate: studies of folate and deoxyribonucleoside triphosphate pools in five mammalian cell lines.

Authors:  M H Tattersall; R C Jackson; S T Jackson; K R Harrap
Journal:  Eur J Cancer       Date:  1974-12       Impact factor: 9.162

6.  Freeze-blowing: a new technique for the study of brain in vivo.

Authors:  R L Veech; R L Harris; D Veloso; E H Veech
Journal:  J Neurochem       Date:  1973-01       Impact factor: 5.372

7.  Control of respiration and metabolism in growing Klebsiella aerogenes. The role of adenine nucleotides.

Authors:  D E Harrison; P K Maitra
Journal:  Biochem J       Date:  1969-05       Impact factor: 3.857

8.  Cell-cycle-dependent variations of deoxyribonucleoside triphosphate pools in Chinese hamster cells.

Authors:  R A Walters; R A Tobey; R L Ratliff
Journal:  Biochim Biophys Acta       Date:  1973-09-07

9.  Deoxyribonucleotide pools during liver regeneration.

Authors:  S S Söderhäll; A Larsson; K L Skoog
Journal:  Eur J Biochem       Date:  1973-02-15

10.  Cyclic adenosine monophosphate in brain areas: microwave irradiation as a means of tissue fixation.

Authors:  M J Schmidt; D E Schmidt; G A Robison
Journal:  Science       Date:  1971-09-17       Impact factor: 47.728
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