Monoclonal antibodies to angiotensin II.

Three hybridomas producing monoclonal antibodies to angiotensin II were generated using somatic cell fusion techniques. Dissociation constants, determined by Scatchard analysis of radioimmunoassay data, were 9.3 X 10(-10) M, 1.5 X 10(-9) M and 1.8 X 10(-8) M for antibodies KAA8, ICH2 and ICA10, respectively. The antibodies exhibited various degrees of cross-reactivity towards peptide metabolites of angiotensin II, but minimal cross-reactivity towards antagonists created by substituting the hormone at both the amino and carboxy terminus. In contrast to a rabbit polyclonal angiotensin II antibody preparation, the three monoclonal antibodies competed effectively with angiotensin receptors on rat adrenal cortical microsomes for hormone binding. These antibodies may have broad utility for a number of applications in angiotensin II research.