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Literature DB >> 3819703

Growth of 293 cells in suspension culture.

Abstract

A subline of 293 cells able to grow in suspension culture has been developed by passage of 293 cells through nude mice. This new line, designated 293N3S, grows with a doubling time of approximately 30 h, continues to express adenovirus 5 early region 1 (E1) antigens, and remains permissive for adenovirus 5 host range mutants defective in E1 functions.

Entities: CellLine Species

Mesh：

Substances：
Antigens, Neoplasm

Year: 1987 PMID： 3819703 DOI： 10.1099/0022-1317-68-3-937

Source DB: PubMed Journal: J Gen Virol ISSN： 0022-1317 Impact factor: 3.891

Keyword Cloud
Cited

25 in total

1. Fusion of Large Polypeptides to Human Adenovirus Type 5 Capsid Protein IX Can Compromise Virion Stability and DNA Packaging Capacity.

Authors: Kathy L Poulin; Emily R McFall; Grace Chan; Natacha B Provost; Carin Christou; Adam C Smith; Robin J Parks
Journal: J Virol Date: 2020-08-17 Impact factor: 5.103

2. Continuous production and recovery of recombinant Ca2+ binding receptor from HEK 293 cells using perfusion through a packed bed bioreactor.

Authors: J B Kaufman; G Wang; W Zhang; M A Valle; J Shiloach
Journal: Cytotechnology Date: 2000-07 Impact factor: 2.058

10. Intratumoral injection of an adenovirus expressing interleukin 2 induces regression and immunity in a murine breast cancer model.

Authors: C L Addison; T Braciak; R Ralston; W J Muller; J Gauldie; F L Graham
Journal: Proc Natl Acad Sci U S A Date: 1995-08-29 Impact factor: 11.205

Growth of 293 cells in suspension culture.

1. Fusion of Large Polypeptides to Human Adenovirus Type 5 Capsid Protein IX Can Compromise Virion Stability and DNA Packaging Capacity.

2. Continuous production and recovery of recombinant Ca2+ binding receptor from HEK 293 cells using perfusion through a packed bed bioreactor.

3. Cloning and characterization of Ras-GRF2, a novel guanine nucleotide exchange factor for Ras.

4. Assembly of helper-dependent adenovirus DNA into chromatin promotes efficient gene expression.

Review 5. Adenovirus-mediated gene transfer into striated muscles.

Review 6. Recent advances in mammalian protein production.

7. Methods for construction of adenovirus vectors.

8. Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells.

9. Low-dose radiation enhances survivin-mediated virotherapy against malignant glioma stem cells.

10. Intratumoral injection of an adenovirus expressing interleukin 2 induces regression and immunity in a murine breast cancer model.