Literature DB >> 3818931

ATP measurements obtained by luminometry provide rapid estimation of Ureaplasma urealyticum growth.

M E Stemler, G W Stemke, J A Robertson.   

Abstract

ATP content obtained by luciferin-luciferase luminometry with commercially available reagents provided rapid estimates of Ureaplasma urealyticum populations. Each cell contained about 4.7 X 10(-18) mol of ATP. We could detect 10(4) CCU50 (color change unit50) per 100 microliters. We correlated urease activity with growth and confirmed the differential response of ureaplasma strains to Mn2+.

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Year:  1987        PMID： 3818931      PMCID： PMC265915          DOI： 10.1128/jcm.25.2.427-429.1987

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

1.  Firefly luminescence in the study of energy transfer mechanisms. I. Substrate and enzyme determination.

Authors:  B L STREHLER; J R TOTTER
Journal:  Arch Biochem Biophys       Date:  1952-09       Impact factor: 4.013

2.  Single-step purification of urease by affinity chromatography.

Authors:  B L Wong; C R Shobe
Journal:  Can J Microbiol       Date:  1974-04       Impact factor: 2.419

3.  Evaluation of a commercially available semi-automated bioluminescence system for bacteriuria screening.

Authors:  P W McWalter; C A Sharp
Journal:  Eur J Clin Microbiol       Date:  1982-08       Impact factor: 3.267

4.  Comparison of two methods for enumeration of mycoplasmas.

Authors:  G W Stemke; J A Robertson
Journal:  J Clin Microbiol       Date:  1982-11       Impact factor: 5.948

5.  [Rapid determination of the minimal inhibitory concentration of an antibiotic by measuring bacterial ATP (author's transl)].

Authors:  J L Fauchère; M Simonet; P Descamps; M Véron
Journal:  Ann Microbiol (Paris)       Date:  1982 Mar-Apr

6.  Effects of manganese on the growth and morphology of Ureaplasma urealyticum.

Authors:  J A Robertson; M H Chen
Journal:  J Clin Microbiol       Date:  1984-06       Impact factor: 5.948

7.  Determination of susceptibility of Staphylococcus aureus to methicillin by luciferin-luciferase assay of bacterial adenosine triphosphate.

Authors:  P W McWalter
Journal:  J Appl Bacteriol       Date:  1984-02

8.  Morphology of the cells and colonies of Mycoplasma hominis.

Authors:  J A Robertson; M Alfa; E S Boatman
Journal:  Sex Transm Dis       Date:  1983 Oct-Dec       Impact factor: 2.830

9.  Synthesis of adenylate nucleotides by Mollicutes (mycoplasmas).

Authors:  K D Beaman; J D Pollack
Journal:  J Gen Microbiol       Date:  1983-10

10.  Rapid diagnosis of bacteraemia by measuring bacterial adenosine triphosphate in blood culture bottles using bioluminescence.

Authors:  B Beckers; H R Lang
Journal:  Med Microbiol Immunol       Date:  1983       Impact factor: 3.402
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  2 in total

1.  Hydrolysis of urea by Ureaplasma urealyticum generates a transmembrane potential with resultant ATP synthesis.

Authors:  D G Smith; W C Russell; W J Ingledew; D Thirkell
Journal:  J Bacteriol       Date:  1993-06       Impact factor: 3.490

2.  Polymerase chain reaction using 16S rRNA gene sequences distinguishes the two biovars of Ureaplasma urealyticum.

Authors:  J A Robertson; A Vekris; C Bebear; G W Stemke
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948
  2 in total

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