Literature DB >> 3814606

Isolation and structure analysis of bee venom mast cell degranulating peptide.

E M Dotimas, K R Hamid, R C Hider, U Ragnarsson.   

Abstract

The venom of Apis mellifera was processed by gel permeation chromatography on Sephadex G-50 and by reversed-phase HPLC. The initial gel permeation step was carried out in the presence of phosphate ions (0.5 M). Ion pair reagents were required to resolve the strongly basic peptides, secapin, mast cell degranulating (MCD-) peptide and apamin, by reversed-phase (RP) HPLC. Using this relatively simple procedure it is possible to isolate these peptides essentially free of melittin (less than 1 in 10(7)) and phospholipase (less than 1 in 10(5] in high yield. The CD spectrum and secondary structure analysis are reported for MCD-peptide and on this basis a solution structure is proposed for this toxin.

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Year:  1987        PMID： 3814606     DOI： 10.1016/0167-4838(87)90069-0

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  7 in total

Review 1.  Immunology of Bee Venom.

Authors:  Daniel Elieh Ali Komi; Farzaneh Shafaghat; Ricardo D Zwiener
Journal:  Clin Rev Allergy Immunol       Date:  2018-06       Impact factor: 8.667

2.  Mast cell degranulating (MCD) peptide analogs with reduced ring structure.

Authors:  A Buku; J Reibman; A Pistelli; P Blandina; D Gazis
Journal:  J Protein Chem       Date:  1992-06

3.  Towards therapeutic applications of arthropod venom k(+)-channel blockers in CNS neurologic diseases involving memory acquisition and storage.

Authors:  Christiano D C Gati; Márcia R Mortari; Elisabeth F Schwartz
Journal:  J Toxicol       Date:  2012-06-04

Review 4.  Three valuable peptides from bee and wasp venoms for therapeutic and biotechnological use: melittin, apamin and mastoparan.

Authors:  Miguel Moreno; Ernest Giralt
Journal:  Toxins (Basel)       Date:  2015-04-01       Impact factor: 4.546

Review 5.  Bee Updated: Current Knowledge on Bee Venom and Bee Envenoming Therapy.

Authors:  Manuela B Pucca; Felipe A Cerni; Isadora S Oliveira; Timothy P Jenkins; Lídia Argemí; Christoffer V Sørensen; Shirin Ahmadi; José E Barbosa; Andreas H Laustsen
Journal:  Front Immunol       Date:  2019-09-06       Impact factor: 7.561

6.  Proteome and phosphoproteome analysis of honeybee (Apis mellifera) venom collected from electrical stimulation and manual extraction of the venom gland.

Authors:  Rongli Li; Lan Zhang; Yu Fang; Bin Han; Xiaoshan Lu; Tiane Zhou; Mao Feng; Jianke Li
Journal:  BMC Genomics       Date:  2013-11-07       Impact factor: 3.969

Review 7.  Current Advances in Immunological Studies on the Vespidae Venom Antigen 5: Therapeutic and Prophylaxis to Hypersensitivity Responses.

Authors:  Murilo Luiz Bazon; Lais Helena Silveira; Patricia Ucelli Simioni; Márcia Regina Brochetto-Braga
Journal:  Toxins (Basel)       Date:  2018-07-24       Impact factor: 4.546
  7 in total

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