Literature DB >> 3814127

Effect of removal of the cytolytic factor of Bacillus thuringiensis subsp. israelensis on mosquito toxicity.

G A Held, Y S Huang, C Y Kawanishi.   

Abstract

Solubilized crystal protein of Bacillus thuringiensis subsp. israelensis was fractionated by affinity chromatography using a monoclonal antibody directed against the crystal's 28 kDa peptide. The 28 kDa peptide was found to be relatively nontoxic to mosquito larvae although it does contain the hemolytic activity of the crystals. The crystal protein fraction depleted of the 28 kDa peptide was found to be nonhemolytic and to retain nearly full toxicity to mosquito larvae. These results suggest that the 28 kDa peptide is not required for the toxicity of solubilized crystal protein.

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Year:  1986        PMID： 3814127     DOI： 10.1016/s0006-291x(86)80133-4

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

1.  Molecular characterization of a gene encoding a 72-kilodalton mosquito-toxic crystal protein from Bacillus thuringiensis subsp. israelensis.

Authors:  W P Donovan; C Dankocsik; M P Gilbert
Journal:  J Bacteriol       Date:  1988-10       Impact factor: 3.490

2.  Deletion by in vivo recombination shows that the 28-kilodalton cytolytic polypeptide from Bacillus thuringiensis subsp. israelensis is not essential for mosquitocidal activity.

Authors:  A Delécluse; J F Charles; A Klier; G Rapoport
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

3.  The Bacillus thuringiensis cyt genes for hemolytic endotoxins constitute a gene family.

Authors:  A Guerchicoff; A Delécluse; C P Rubinstein
Journal:  Appl Environ Microbiol       Date:  2001-03       Impact factor: 4.792

4.  Toxicity of protease-resistant domains from the delta-endotoxin of Bacillus thuringiensis subsp. israelensis in Culex quinquefasciatus and Aedes aegypti bioassays.

Authors:  M A Pfannenstiel; W C Cray; G A Couche; K W Nickerson
Journal:  Appl Environ Microbiol       Date:  1990-01       Impact factor: 4.792

5.  Affinity purification of a 65-kilodalton parasporal protein from Bacillus thuringiensis PG-14 that shows mosquitocidal activity.

Authors:  Y M Yu; M Ohba; K Aizawa
Journal:  Antonie Van Leeuwenhoek       Date:  1988       Impact factor: 2.271

6.  Binding of Cyt1Aa and Cry11Aa toxins of Bacillus thuringiensis serovar israelensis to brush border membrane vesicles of Tipula paludosa (Diptera: Nematocera) and subsequent pore formation.

Authors:  Jesko Oestergaard; Ralf-Udo Ehlers; Amparo C Martínez-Ramírez; Maria Dolores Real
Journal:  Appl Environ Microbiol       Date:  2007-04-06       Impact factor: 4.792

7.  Characterization of the parasporal inclusion of Bacillus thuringiensis subsp. kyushuensis.

Authors:  G A Held; C Y Kawanishi; Y S Huang
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490
  7 in total

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