Temporary bone tissue ischemia in the hind limb of the rabbit. A vital microscopic study.

With in situ bone vital microscopy it was demonstrated that a cuff pressure of 300 mm Hg applied over the rabbit thigh is sufficient to cause a complete vascular standstill in the tibial cortex. Cuff-induced tissue ischemia applied for 30 min did not result in any adverse bone tissue reactions over a follow-up period of 30 days. Locally applied isolated bone ischemia for 3 or 6h resulted in an inflammatory response, and 4-6 weeks after the 6-h ischemia bone resorption was evident.