Literature DB >> 3812997

High-performance liquid chromatography and ultraviolet spectrophotometry for quantitation of tryptophan in barytic hydrolysates.

S Delhaye, J Landry.   

Abstract

A procedure for quantitation of tryptophan in feedstuffs is described. It is based on barytic hydrolysis of material at 125 degrees C for 16 h, acidification of hydrolysate to pH 3 with HCl, high-performance liquid chromatography on Nova Pak C18 (Waters Assoc.), and spectrophotometric determination of tryptophan at 280 nm. The recovery of tryptophan from lysozyme added to samples ranges from 98.7 to 100%.

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Year:  1986        PMID： 3812997     DOI： 10.1016/0003-2697(86)90324-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  Biosynthesis and characterization of 4-fluorotryptophan-labeled Escherichia coli arginyl-tRNA synthetase.

Authors:  Q S Zhang; L Shen; E D Wang; Y L Wang
Journal:  J Protein Chem       Date:  1999-02

2.  Manipulation of amino acid composition in soybean seeds by the combination of deregulated tryptophan biosynthesis and storage protein deficiency.

Authors:  Yoichi Kita; Yumi Nakamoto; Masakazu Takahashi; Keisuke Kitamura; Kyo Wakasa; Masao Ishimoto
Journal:  Plant Cell Rep       Date:  2009-11-27       Impact factor: 4.570

3.  HPLC determination of tryptophan in foodstuffs using barium hydroxide hydrolysis at 140°C.

Authors:  J Landry; S Delhaye
Journal:  Amino Acids       Date:  1993-06       Impact factor: 3.520
  3 in total

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