| Literature DB >> 3812971 |
S Yamato, I Sahara, H Sugihara, K Shimada.
Abstract
A spectrophotometric method for inorganic phosphate determination in the presence of thiol compounds is described. Thiol compounds, which interfere with the measurement of inorganic phosphate by a modification of the method of Gomori, are removed by carboxymethylation by iodoacetate prior to the formation and reduction of phosphomolybdate complex. A linear standard curve is obtained by this method, and the method is suitable for the assay of a phosphate-releasing enzyme when the measurement must be performed in the presence of thiol compounds.Entities:
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Year: 1986 PMID： 3812971 DOI： 10.1016/0003-2697(86)90548-8
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365