Literature DB >> 3812968

Recovery of nanogram quantities of DNA from plasma and quantitative measurement using labeling by nick translation.

G J Fournié, M Gayral-Taminh, J P Bouché, J J Conté.   

Abstract

A method which allows for the quantitative measurement of DNA in plasma is described. After treatment of plasma with phenol, DNA is precipitated by ethanol using gelatin as a coprecipitating agent. DNA is then measured by nick translation labeling. This assay takes a few hours. It is suitable for the measurement of DNA within a range of 0.02 to 20 ng in 10 microliters of plasma. For example, it is applied to the measurement of DNA in plasma from mice injected with bacterial lipopolysaccharide.

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Year:  1986        PMID： 3812968     DOI： 10.1016/0003-2697(86)90545-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

1.  Antigen DNA isolated from immune complexes in plasma of patients with systemic lupus erythematosus hybridizes with the Escherichia coli lac Z gene.

Authors:  K Terada; E Okuhara; Y Kawarada
Journal:  Clin Exp Immunol       Date:  1991-07       Impact factor: 4.330

2.  Liquid Biopsy in Breast Cancer: Circulating Tumor Cells and Circulating Tumor DNA.

Authors:  Tae-Kyung Yoo
Journal:  Adv Exp Med Biol       Date:  2021       Impact factor: 2.622

Review 3.  Analysis of circulating tumor DNA in breast cancer as a diagnostic and prognostic biomarker.

Authors:  Mersedeh Rohanizadegan
Journal:  Cancer Genet       Date:  2018-02-24

4.  In vitro inhibition of natural-killer-mediated lysis by chromatin fragments.

Authors:  A D Le Lann; G J Fournié; L Boissier; P L Toutain; H Benoist
Journal:  Cancer Immunol Immunother       Date:  1994-09       Impact factor: 6.968

5.  Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation.

Authors:  Alexandra S Whale; Jim F Huggett; Simon Cowen; Valerie Speirs; Jacqui Shaw; Stephen Ellison; Carole A Foy; Daniel J Scott
Journal:  Nucleic Acids Res       Date:  2012-02-28       Impact factor: 16.971

6.  Detection of mutant K-ras DNA in plasma or serum of patients with colorectal cancer.

Authors:  M S Kopreski; F A Benko; C Kwee; K E Leitzel; E Eskander; A Lipton; C D Gocke
Journal:  Br J Cancer       Date:  1997       Impact factor: 7.640

Review 7.  Advances in the Detection Technologies and Clinical Applications of Circulating Tumor DNA in Metastatic Breast Cancer.

Authors:  Hao Liao; Huiping Li
Journal:  Cancer Manag Res       Date:  2020-05-18       Impact factor: 3.989
  7 in total

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