| Literature DB >> 381197 |
Abstract
A Streptococcus agalactiae isolate of bovine origin was cultured in broth; log-phase cells were washed and radioiodinated and subsequently extracted at low pH in the presence of a nonionic detergent. A protein antigen was purified from concentrated extract by ultracentrifugation, gel filtration, and ion-exchange chromatography. The molecular weight of the protein was estimated at 31,800. The agglutinogenic character of the protein indicated its localization at the cell surface.Entities:
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Year: 1979 PMID: 381197 PMCID: PMC414353 DOI: 10.1128/iai.24.3.637-641.1979
Source DB: PubMed Journal: Infect Immun ISSN: 0019-9567 Impact factor: 3.441