| Literature DB >> 380640 |
G Dooijewaard, F F Roossien, G T Robillard.
Abstract
A rapid, high-yield procedure has been developed for the purification of HPr from the Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. During this procedure, the protein copurifies with a 2500-dalton homopolysaccharide which we have identified as alpha 1-6 glucan. The results of steady-state kinetic measurements of the phosphotransferase activity demonstrate that the polysaccharide works as an activator of the phosphotransferase system probably at the level of the HPr:P-E1 complex or the P-HPr:E11 complex.Entities:
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Year: 1979 PMID： 380640 DOI： 10.1021/bi00581a013
Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162