| Literature DB >> 3805787 |
G M Bressan, I Pasquali-Ronchetti, C Fornieri, F Mattioli, I Castellani, D Volpin.
Abstract
Solutions of tropoelastin incubated under different experimental conditions were examined by electron microscopy after negative staining and after fixation and embedding. Below 37 degrees C only polymorphous structureless elements of variable size could be found. In samples kept for a few minutes at 40 degrees C, flexible, isolated filaments of 5 nm diameter and variable length, together with a few small aggregates of filaments, were seen. No single filaments, but only bundles of filaments were detectable after incubation at 40 degrees C for longer than 5-10 min. Tropoelastin kept at 40 degrees C for longer than 10 hr formed a white precipitate, which, when fixed and embedded as in conventional electron microscopy, consisted of 0.5-2 microns thick, amorphous and branching fibers, identical to those seen in identically processed normal tissues. From these observations a model for the assembly and structure of elastic fibers is proposed.Entities:
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Year: 1986 PMID： 3805787 DOI： 10.1016/0889-1605(86)90068-6
Source DB: PubMed Journal: J Ultrastruct Mol Struct Res ISSN： 0889-1605