Production of monoclonal antibodies. The effect of hybridoma concentration on the yield of antibody-producing clones.

The relationship between the number of hybrid clones in each well of the primary culture, the yield of antibody-producing wells and the short-term stability of the polyclonal cultures was tested. The results of the study demonstrate that a higher yield of antibody-secreting clones was obtained by employing lower density of hybrids and that instability of antibody secretion by polyclonal cultures is not the result of overgrowth of non-secreting clones.