| Literature DB >> 3804606 |
H Biesalski, H Greiff, K Brodda, G Hafner, K H Bässler.
Abstract
The simultaneous determination of alpha-tocopherol and retinol in human serum is reported. The separation is carried out by means of isocratic HPLC on adsorption columns. UV-Detection is possible by using either one wavelength for both compounds (300 nm), or after a lambda change mode with typical wavelengths for alpha-tocopherol (292 nm) and retinol (325 nm). According to short retention times (10 min) and rapid extraction the method is useful for clinical research and allows about 50 analyses per day and operator. Blood from 176 human volunteers was collected and alpha-tocopherol and retinol levels in serum determined with this method. Statistical evaluation of different selected groups shows typical significant differences of alpha-tocopherol and retinol concentrations in smokers and oral contraceptive users.Entities:
Keywords: Behavior; Biology; Clinical Research; Contraception; Contraceptive Agents; Contraceptive Agents, Female; Contraceptive Methods--side effects; Economic Factors; Examinations And Diagnoses; Family Planning; Laboratory Examinations And Diagnoses; Laboratory Procedures; Oral Contraceptives--side effects; Physiology; Research And Development; Research Methodology; Smoking--side effects; Social Behavior; Technology; Vitamin A; Vitamins
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Year: 1986 PMID： 3804606
Source DB: PubMed Journal: Int J Vitam Nutr Res ISSN： 0300-9831 Impact factor: 1.784