Literature DB >> 3803348

Computer analysis of enzyme-substrate-inhibitor kinetic data with automatic model selection using IBM-PC compatible microcomputers.

R A Lutz, C Bull, D Rodbard.   

Abstract

A weighted nonlinear least-squares curve-fitting program, implemented in compiled BASIC for the IBM-PC is described to estimate the parameters of enzyme kinetics obeying Michaelis-Menten kinetics and seven inhibition models. The effects of the inhibitor on the maximal velocity (Vm) and the Michaelis-Menten constant (Km) are used to select automatically the most plausible model of inhibition and to calculate initial estimates of parameters. The program is used to demonstrate that the inhibition of carbamyl-phenylalanine hydrolase by the product phenylalanine is consistent with the pure mixed noncompetitive model.

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Year:  1986        PMID： 3803348     DOI： 10.1159/000469292

Source DB:  PubMed          Journal:  Enzyme        ISSN： 0013-9432


  10 in total

1.  Identification of a specific region of low molecular weight phospholipases A2 (residues 21-40) as a potential target for structure-based design of inhibitors of these enzymes.

Authors:  E Cordella-Miele; L Miele; A B Mukherjee
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-01       Impact factor: 11.205

2.  Pea formaldehyde-active class III alcohol dehydrogenase: common derivation of the plant and animal forms but not of the corresponding ethanol-active forms (classes I and P).

Authors:  J Shafqat; M El-Ahmad; O Danielsson; M C Martínez; B Persson; X Parés; H Jornvall
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-28       Impact factor: 11.205

3.  Analysis of progress curves by simulations generated by numerical integration.

Authors:  C T Zimmerle; C Frieden
Journal:  Biochem J       Date:  1989-03-01       Impact factor: 3.857

4.  Uptake of norepinephrine and related catecholamines by cultured chromaffin cells: characterization of cocaine-sensitive and -insensitive plasma membrane transport sites.

Authors:  D K Banerjee; R A Lutz; M A Levine; D Rodbard; H B Pollard
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205

5.  Design of an adenosine phosphorylase by active-site modification of murine purine nucleoside phosphorylase. Enzyme kinetics and molecular dynamics simulation of Asn-243 and Lys-244 substitutions of purine nucleoside phosphorylase.

Authors:  J T Maynes; W Yam; J P Jenuth; R Gang Yuan; S A Litster; B M Phipps; F F Snyder
Journal:  Biochem J       Date:  1999-12-01       Impact factor: 3.857

6.  Functional analysis of the mutations in the human cardiac beta-myosin that are responsible for familial hypertrophic cardiomyopathy. Implication for the clinical outcome.

Authors:  M Sata; M Ikebe
Journal:  J Clin Invest       Date:  1996-12-15       Impact factor: 14.808

7.  The vertebrate alcohol dehydrogenase system: variable class II type form elucidates separate stages of enzymogenesis.

Authors:  L Hjelmqvist; M Estonius; H Jörnvall
Journal:  Proc Natl Acad Sci U S A       Date:  1995-11-21       Impact factor: 11.205

8.  Hypoxanthine-guanine phosphoribosyltransferase deficiency in three brothers with gout: characterization of a variant, HPRTEdinburgh, having altered isoelectric point, increased thermal lability and normal levels of messenger RNA.

Authors:  F F Snyder; J E Joyce; T Carter-Edwards; R Joshi; H L Rylance; R C Wallace; G Nuki
Journal:  J Inherit Metab Dis       Date:  1989       Impact factor: 4.982

9.  Mutation of Arg-115 of human class III alcohol dehydrogenase: a binding site required for formaldehyde dehydrogenase activity and fatty acid activation.

Authors:  K Engeland; J O Höög; B Holmquist; M Estonius; H Jörnvall; B L Vallee
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-15       Impact factor: 11.205

10.  YMXM motifs of IRS-1 define substrate specificity of the insulin receptor kinase.

Authors:  S E Shoelson; S Chatterjee; M Chaudhuri; M F White
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-15       Impact factor: 11.205
  10 in total

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