Literature DB >> 3799971

A sensitive bioluminescence assay for myo-inositol.

T W Gudermann, T G Cooper.   

Abstract

A specific, sensitive bioluminescence assay for myo-inositol is described employing myo-inositol dehydrogenase linked to a commercial NADH bioluminescence kit. Optimum conditions providing a linear response over 4 orders of magnitude are presented with reproducibility of 6% CV and a sensitivity of 1 pmol. The assay is suitable for the measurement of inositol at low concentrations or in small volumes.

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Year:  1986        PMID： 3799971     DOI： 10.1016/0003-2697(86)90588-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  Measurement of picomole amounts of any inositol phosphate isomer separable by h.p.l.c. by means of a bioluminescence assay.

Authors:  S A Prestwich; T B Bolton
Journal:  Biochem J       Date:  1991-03-15       Impact factor: 3.857

2.  Comparison of the levels of inositol metabolites in transformed haemopoietic cells and their normal counterparts.

Authors:  C M Bunce; P J French; P Allen; J C Mountford; B Moor; M F Greaves; R H Michell; G Brown
Journal:  Biochem J       Date:  1993-02-01       Impact factor: 3.857

3.  SAC1p is an integral membrane protein that influences the cellular requirement for phospholipid transfer protein function and inositol in yeast.

Authors:  E A Whitters; A E Cleves; T P McGee; H B Skinner; V A Bankaitis
Journal:  J Cell Biol       Date:  1993-07       Impact factor: 10.539
  3 in total

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