Literature DB >> 3796235

An improved method for the separation of molecular species of cerebrosides.

Y Hirabayashi, A Hamaoka, M Matsumoto, K Nishimura.   

Abstract

A method employing reversed-phase high performance liquid chromatography (HPLC) has been developed for the analysis of molecular species of underivatized cerebrosides. By using a high carbon (20%) C18-silica bead column, monohexosylceramides isolated from bovine brain and Japanese quail intestine could each be separated into 42 and 56 peaks, respectively, and could subsequently be recovered. Under our analytical conditions, underivatized cerebrosides with 24:1 and 22:0 fatty acids were completely separated by single step HPLC. Each of the molecular species was then analyzed by high performance thin layer chromatography and gas chromatography-mass spectrometry (or fast atom bombardment mass spectrometry). This analytical method permits the complete identification of molecular species of glycosphingolipids.

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Year:  1986        PMID： 3796235     DOI： 10.1007/bf02537245

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  10 in total

1.  Comparative study on glycolipid composition between two cell types of rat ascites hepatoma cells.

Authors:  Y Hirabayashi; T Taki; M Matsumoto; K Kojima
Journal:  Biochim Biophys Acta       Date:  1978-04-28

2.  Separation of molecular species of sphingomyelin and ceramide by argentation and reversed-phase HPLC.

Authors:  M Smith; P Monchamp; F B Jungalwala
Journal:  J Lipid Res       Date:  1981-05       Impact factor: 5.922

3.  Separation of molecular species of sphingomyelin by reversed-phase high-performance liquid chromatography.

Authors:  F B Jungalwala; V Hayssen; J M Pasquini; R H McCluer
Journal:  J Lipid Res       Date:  1979-07       Impact factor: 5.922

4.  Developmental profiles of glycolipids in mouse small intestine.

Authors:  E Sato; T Uezato; M Fujita; K Nishimura
Journal:  J Biochem       Date:  1982-06       Impact factor: 3.387

5.  High performance liquid chromatographic analysis of long chain bases in intestinal glycolipids of adult and embryonic Japanese quails.

Authors:  K Nishimura; A Nakamura
Journal:  J Biochem       Date:  1985-11       Impact factor: 3.387

6.  An improved procedure for the quantitative determination and characterization of sulfatides in rat kidney and brain by high-performance liquid chromatography.

Authors:  K Shimomura; Y Kishimoto
Journal:  Biochim Biophys Acta       Date:  1983-11-01

7.  Molecular species of monoglycosylceramides in embryonic and adult Japanese quail intestine.

Authors:  K Nishimura; Y Hirabayashi; A Hamaoka; M Matsumoto; A Nakamura; K Miseki
Journal:  Biochim Biophys Acta       Date:  1984-12-06

8.  Reverse phase high-performance liquid chromatography of cerebrosides,sulfatides, and ceramides: microanalysis of homolog composition without hydrolysis and application to cerebroside analysis in peripheral nerves of adrenoleukodystrophy pateints.

Authors:  S Yahara; H W Moser; E H Kolodny; Y Kishimoto
Journal:  J Neurochem       Date:  1980-03       Impact factor: 5.372

9.  Simultaneous determination of picomole levels of gluco- and galactocerebroside, monogalactosyl diglyceride, and sulfatide by high performance liquid chromatography.

Authors:  G Nonaka; Y Kishimoto
Journal:  Biochim Biophys Acta       Date:  1979-03-29

10.  High performance liquid chromatography preparation of the molecular species of GM1 and GD1a gangliosides with homogeneous long chain base composition.

Authors:  S Sonnino; R Ghidoni; G Gazzotti; G Kirschner; G Galli; G Tettamanti
Journal:  J Lipid Res       Date:  1984-06       Impact factor: 5.922
  10 in total
  8 in total

1.  An improved method for analysis of glucosylceramide species having cis-8 and trans-8 isomers of sphingoid bases by LC-MS/MS.

Authors:  Hiroyuki Imai; Hideyasu Hattori; Masayuki Watanabe
Journal:  Lipids       Date:  2012-10-30       Impact factor: 1.880

2.  Subcellular biosynthesis and transport of gangliosides formed from exogenous lactosylceramide in rat liver.

Authors:  M Trinchera; R Ghidoni
Journal:  Biochem J       Date:  1990-03-01       Impact factor: 3.857

3.  Analysis of Glucocerebrosides of Rye (Secale cereale L. cv Puma) Leaf and Plasma Membrane.

Authors:  E B Cahoon; D V Lynch
Journal:  Plant Physiol       Date:  1991-01       Impact factor: 8.340

4.  Recycling of glucosylceramide and sphingosine for the biosynthesis of gangliosides and sphingomyelin in rat liver.

Authors:  M Trinchera; R Ghidoni; S Sonnino; G Tettamanti
Journal:  Biochem J       Date:  1990-09-15       Impact factor: 3.857

5.  Synthesis, NMR characterization and divergent biological actions of 2'-hydroxy-ceramide/dihydroceramide stereoisomers in MCF7 cells.

Authors:  Zdzislaw M Szulc; Aiping Bai; Jacek Bielawski; Nalini Mayroo; Doreen E Miller; Hanna Gracz; Yusuf A Hannun; Alicja Bielawska
Journal:  Bioorg Med Chem       Date:  2010-09-28       Impact factor: 3.641

6.  An extended method for separating and quantitating molecular species of phospholipids.

Authors:  M G Wiley; M Przetakiewicz; M Takahashi; J M Lowenstein
Journal:  Lipids       Date:  1992-04       Impact factor: 1.880

7.  Shotgun glycomics: a microarray strategy for functional glycomics.

Authors:  Xuezheng Song; Yi Lasanajak; Baoyun Xia; Jamie Heimburg-Molinaro; Jeanne M Rhea; Hong Ju; Chunmei Zhao; Ross J Molinaro; Richard D Cummings; David F Smith
Journal:  Nat Methods       Date:  2010-12-05       Impact factor: 28.547

8.  Malassezia and Parkinson's Disease.

Authors:  Martin Laurence; Julián Benito-León; Frédéric Calon
Journal:  Front Neurol       Date:  2019-07-24       Impact factor: 4.003
  8 in total

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