The secondary spikes of climbing fibre responses recorded from Purkinje cell axons in cat cerebellum.

Responses evoked in Purkinje cells by climbing fibre activity were investigated by recording from Purkinje cell axons in the cerebellum of anaesthetized cats. Purkinje cell axons were identified by firing pattern and by latency of responses to stimulation of peripheral nerve and of the inferior olive. Axonal climbing fibre responses usually consisted of one to two spikes, suggesting that normally only the initial spike or, at most, this and one of the secondary spikes are propagated down the Purkinje cell axon. When two successive climbing fibre responses were evoked, the number of spikes in the second response was increased, usually up to three to five. This effect could be obtained at stimulation intervals of up to 100 ms. In a few cases it was possible for a climbing fibre response to be preceded by a parallel fibre volley evoked by stimulation of the cerebellar surface. This increased the number of spikes in the axonal climbing fibre response. The results suggest that the number of propagated spikes in the climbing fibre response can be modified by a preceding input to the Purkinje cell.