Literature DB >> 3794346

Purification of human monocytes on gelatin-coated surfaces.

N F Hassan, D E Campbell, S D Douglas.   

Abstract

Human peripheral blood monocytes secrete a cell membrane-associated glycoprotein, cold insoluble globulin (fibronectin). Since fibronectin binds to gelatin-coated surface, we developed a simple technique for separation of human peripheral blood monocytes from whole mononuclear cell preparation. These preparations are characterized by a high monocyte purity (more than 90%), low platelet contamination and excellent viability.

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Year:  1986        PMID: 3794346     DOI: 10.1016/0022-1759(86)90415-1

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  59 in total

1.  Monocytes/macrophages in diagnosis and immunopathogenesis.

Authors:  S D Douglas
Journal:  Clin Diagn Lab Immunol       Date:  1999-05

2.  Substance P antagonist (CP-96,345) inhibits HIV-1 replication in human mononuclear phagocytes.

Authors:  J P Lai; W Z Ho; G X Zhan; Y Yi; R G Collman; S D Douglas
Journal:  Proc Natl Acad Sci U S A       Date:  2001-03-27       Impact factor: 11.205

3.  Alpha-defensins inhibit HIV infection of macrophages through upregulation of CC-chemokines.

Authors:  Chang-Jiang Guo; Ning Tan; Li Song; Steven D Douglas; Wen-Zhe Ho
Journal:  AIDS       Date:  2004-05-21       Impact factor: 4.177

Review 4.  Modulation of intracellular restriction factors contributes to methamphetamine-mediated enhancement of acquired immune deficiency syndrome virus infection of macrophages.

Authors:  Xu Wang; Yizhong Wang; Li Ye; Jieliang Li; Yu Zhou; Sinem Sakarcan; Wenzhe Ho
Journal:  Curr HIV Res       Date:  2012-07       Impact factor: 1.581

5.  Cellular microRNA expression correlates with susceptibility of monocytes/macrophages to HIV-1 infection.

Authors:  Xu Wang; Li Ye; Wei Hou; Yu Zhou; Yan-Jian Wang; David S Metzger; Wen-Zhe Ho
Journal:  Blood       Date:  2008-11-17       Impact factor: 22.113

6.  An in vitro model of morphine withdrawal manifests the enhancing effect on human immunodeficiency virus infection of human T lymphocytes through the induction of substance P.

Authors:  Xu Wang; Steven D Douglas; Jin-Song Peng; Dun-Jin Zhou; Qi Wan; Wen-Zhe Ho
Journal:  Am J Pathol       Date:  2006-11       Impact factor: 4.307

7.  Morphine enhances HIV infection of neonatal macrophages.

Authors:  Yuan Li; Jeffrey D Merrill; Kathy Mooney; Li Song; Xu Wang; Chang-Jiang Guo; Rashmin C Savani; David S Metzger; Steven D Douglas; Wen-Zhe Ho
Journal:  Pediatr Res       Date:  2003-05-07       Impact factor: 3.756

8.  Methamphetamine enhances HIV infection of macrophages.

Authors:  Hao Liang; Xu Wang; Hui Chen; Li Song; Li Ye; Shi-Hong Wang; Yan-Jian Wang; Lin Zhou; Wen-Zhe Ho
Journal:  Am J Pathol       Date:  2008-05-05       Impact factor: 4.307

Review 9.  Assessing identity, phenotype, and fate of endothelial progenitor cells.

Authors:  Karen K Hirschi; David A Ingram; Mervin C Yoder
Journal:  Arterioscler Thromb Vasc Biol       Date:  2008-07-31       Impact factor: 8.311

10.  Morphine suppresses intracellular interferon-alpha expression in neuronal cells.

Authors:  Qi Wan; Xu Wang; Yan-Jian Wang; Li Song; Shi-Hong Wang; Wen-Zhe Ho
Journal:  J Neuroimmunol       Date:  2008-06-17       Impact factor: 3.478

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