| Literature DB >> 379234 |
A G Harmsen, J R Birmingham, R L Engen, E L Jeska.
Abstract
A technique for obtaining alveolar macrophages (AM) from anesthetized swine is described. Animals were intubated and segmental pulmonary lavage was performed utilizing a double lumen catheter (DLC). An average of 98% of the initial 100 ml lavage fluid was recovered with a typical yield of 1 x 10(8) free alveolar cells (FAC). AM were then separated from other FAC by their adherence to plastic. The final adherent cell population consisted of greater than 95% macrophage as determined by morphology and non-specific esterase activity. The technique described had no adverse effects on the animals even when repeated on the same animal several days later.Entities:
Mesh：
Year: 1979 PMID： 379234 DOI： 10.1016/0022-1759(79)90265-5
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303