Literature DB >> 3783815

The bacteriophage T4 regulatory protein gpunf/alc binds to DNA in the absence of RNA polymerase.

D P Snustad, N Haas, D G Oppenheimer.   

Abstract

DNA-cellulose chromatography and two-dimensional gel electrophoresis have been used to demonstrate the DNA-binding capacity of bacteriophage T4 gpunf/alc. The unf/alc protein does not bind to DNA via an association with RNA polymerase; gpunf/alc was shown to bind to DNA after separation from RNA polymerase and other large proteins by Sephadex chromatography.

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Year:  1986        PMID： 3783815      PMCID： PMC253370     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  15 in total

1.  A gene of bacteriophage T4 whose product prevents true late transcription on cytosine-containing T4 DNA.

Authors:  L Snyder; L Gold; E Kutter
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205

2.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

3.  Slow switchover from host RNA synthesis to bacteriophage RNA synthesis after infection of Escherichia coli with a T4 mutant defective in the bacteriophage T4-induced unfolding of the host nucleoid.

Authors:  M A Tigges; C J Bursch; D P Snustad
Journal:  J Virol       Date:  1977-12       Impact factor: 5.103

4.  Bacteriophage T4 unf (=alc) gene function is required for late replication in the presence of plasmid pR386.

Authors:  R E Herman; D P Snustad
Journal:  J Virol       Date:  1985-02       Impact factor: 5.103

5.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

6.  Studies with DNA-cellulose chromatography. I. DNA-binding proteins from Escherichia coli.

Authors:  B M Alberts; F J Amodio; M Jenkins; E D Gutmann; F L Ferris
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1968

7.  T4 Bacteriophage-coded RNA polymerase subunit blocks host transcription and unfolds the host chromosome.

Authors:  K Sirotkin; J Wei; L Snyder
Journal:  Nature       Date:  1977-01-06       Impact factor: 49.962

8.  Rapid micromethod for the purification of Escherichia coli ribonucleic acid polymerase and the preparation of bacterial extracts active in ribonucleic acid synthesis.

Authors:  C Gross; F Engbaek; T Flammang; R Burgess
Journal:  J Bacteriol       Date:  1976-10       Impact factor: 3.490

9.  Mutants of bacteriophage T4 deficient in the ability to induce nuclear disruption: shutoff of host DNA and protein synthesis gene dosage experiments, identification of a restrictive host, and possible biological significance.

Authors:  D P Snustad; C J Bursch; K A Parson; S H Hefeneider
Journal:  J Virol       Date:  1976-04       Impact factor: 5.103

10.  Identification and preliminary characterization of a mutant defective in the bacteriophage T4-induced unfolding of the Escherichia coli nucleoid.

Authors:  D P Snustad; M A Tigges; K A Parson; C J Bursch; F M Caron; J F Koerner; D J Tutas
Journal:  J Virol       Date:  1976-02       Impact factor: 5.103
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  2 in total

1.  Inhibition of transcription of cytosine-containing DNA in vitro by the alc gene product of bacteriophage T4.

Authors:  R H Drivdahl; E M Kutter
Journal:  J Bacteriol       Date:  1990-05       Impact factor: 3.490

2.  Escherichia coli mutations that prevent the action of the T4 unf/alc protein map in an RNA polymerase gene.

Authors:  L Snyder; L Jorissen
Journal:  Genetics       Date:  1988-02       Impact factor: 4.562
  2 in total

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