Literature DB >> 3783694

Collagen self-assembly in vitro: electron microscopy of initial aggregates formed during the lag phase.

N P Ward, D J Hulmes, J A Chapman.   

Abstract

Initial aggregates formed in collagen self-assembly were visualized by electron microscopy, using formaldehyde to fix the state of aggregation at various points in the turbidimetric lag phase. Measurements of the length distributions of monomers and small oligomers show that the first-formed aggregates are dimeric, with the most prevalent dimer having a maximal (approximately equal to 4D; D = 67 nm) stagger between constituent molecules.

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Year:  1986        PMID: 3783694     DOI: 10.1016/0022-2836(86)90079-3

Source DB:  PubMed          Journal:  J Mol Biol        ISSN: 0022-2836            Impact factor:   5.469


  5 in total

1.  Helical model of nucleation and propagation to account for the growth of type I collagen fibrils from symmetrical pointed tips: a special example of self-assembly of rod-like monomers.

Authors:  D Silver; J Miller; R Harrison; D J Prockop
Journal:  Proc Natl Acad Sci U S A       Date:  1992-10-15       Impact factor: 11.205

Review 2.  Collagen fibril formation.

Authors:  K E Kadler; D F Holmes; J A Trotter; J A Chapman
Journal:  Biochem J       Date:  1996-05-15       Impact factor: 3.857

3.  Structure-function studies on hsp47: pH-dependent inhibition of collagen fibril formation in vitro.

Authors:  C A Thomson; V S Ananthanarayanan
Journal:  Biochem J       Date:  2000-08-01       Impact factor: 3.857

4.  Sequential assembly of collagen revealed by atomic force microscopy.

Authors:  M Gale; M S Pollanen; P Markiewicz; M C Goh
Journal:  Biophys J       Date:  1995-05       Impact factor: 4.033

5.  Dermatan sulphate proteoglycans from sclera examined by rotary shadowing and electron microscopy.

Authors:  N P Ward; J E Scott; L Cöster
Journal:  Biochem J       Date:  1987-03-15       Impact factor: 3.857

  5 in total

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