| Literature DB >> 3782009 |
P F Smith, G Krack, R L McKee, D G Johnson, A J Gandolfi, V J Hruby, C L Krumdieck, K Brendel.
Abstract
Adult rat liver slices were maintained for 20 h in a novel organ culture system with minimal loss of viability and function. Potassium and adenosine triphosphate levels were maintained at in vivo levels, following an initial recovery period (2 to 4 h), for up to 20 h. Protein synthesis and secretion were linear for 20 and 16 h, respectively. In addition, the liver slices synthesized glycogen between 4 and 12 h in culture. Finally, the liver slices were hormonally responsive during the 20 h culture period as exemplified by glucagon-stimulated glucose production. This system provides a simple and effective method for the culture and biochemical maintenance of adult rat liver for 20 h with minimal loss of biochemical function.Entities:
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Year: 1986 PMID： 3782009 DOI： 10.1007/bf02621087
Source DB: PubMed Journal: In Vitro Cell Dev Biol ISSN： 0883-8364