Literature DB >> 3780374

A vector-primer-cloner-sequencer plasmid for the construction of cDNA libraries: evidence for a rat glyceraldehyde-3-phosphate dehydrogenase-like mRNA and a ferritin mRNA within testis.

S A Krawetz, W Connor, P D Cannon, G H Dixon.   

Abstract

We have developed a vector-primer-cloner-sequencer (VPCS), based on the pUC plasmids, which is easy to prepare. Stable cDNA libraries have been generated from human, rat, and bull testis. The inserts are of various size classes, including high-molecular-weight reverse transcripts which can be easily sequenced. The utility of this VPCS has been demonstrated by isolating a rat ferritin and glyceraldehyde-3-phosphate dehydrogenase-like clone.

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Year:  1986        PMID： 3780374     DOI： 10.1089/dna.1986.5.427

Source DB:  PubMed          Journal:  DNA        ISSN： 0198-0238


  4 in total

Review 1.  Mouse chromosome 19.

Authors:  J L Guénet; M Watson; M F Seldin
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

2.  Cleavage of rRNA ensures translational cessation in sperm at fertilization.

Authors:  G D Johnson; E Sendler; C Lalancette; R Hauser; M P Diamond; S A Krawetz
Journal:  Mol Hum Reprod       Date:  2011-08-10       Impact factor: 4.025

3.  Hypusine modification in eukaryotic initiation factor 5A in rodent cells selected for resistance to growth inhibition by ornithine decarboxylase-inhibiting drugs.

Authors:  M E Tome; E W Gerner
Journal:  Biochem J       Date:  1996-11-15       Impact factor: 3.857

4.  Expression of an insulin/interleukin-1 receptor antagonist hybrid gene in insulin-producing cell lines (HIT-T15 and NIT-1) confers resistance against interleukin-1-induced nitric oxide production.

Authors:  N Welsh; K Bendtzen; M Welsh
Journal:  J Clin Invest       Date:  1995-04       Impact factor: 14.808
  4 in total

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