Literature DB >> 3780372

A system for the simultaneous chemical synthesis of different DNA fragments on solid support.

W Bannwarth, P Iaiza.   

Abstract

A mechanical apparatus for the simultaneous synthesis of different DNA fragments on solid support is described. This new system allows the use of different kinds of supports and is amenable to automation. Utilizing this apparatus the synthesis of DNA fragments can be speeded up considerably. The effectiveness of the system is demonstrated by the simultaneous synthesis of DNA fragments up to 36 nucleotides long using phosphoamidites as building blocks and controlled pore glass as solid support.

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Year:  1986        PMID： 3780372     DOI： 10.1089/dna.1986.5.413

Source DB:  PubMed          Journal:  DNA        ISSN： 0198-0238


  4 in total

1.  More than 150 nucleotides flanking the initiation codon contribute to the efficiency of the ribosomal binding site from bacteriophage T7 gene 1.

Authors:  R M Geisen; H P Fatscher; E Fuchs
Journal:  Nucleic Acids Res       Date:  1987-06-25       Impact factor: 16.971

2.  An unstructured mRNA region and a 5' hairpin represent important elements of the E. coli translation initiation signal determined by using the bacteriophage T7 gene 1 translation start site.

Authors:  A Helke; R M Geisen; M Vollmer; M L Sprengart; E Fuchs
Journal:  Nucleic Acids Res       Date:  1993-12-11       Impact factor: 16.971

3.  HIV-1 RT-associated ribonuclease H displays both endonuclease and 3'----5' exonuclease activity.

Authors:  O Schatz; J Mous; S F Le Grice
Journal:  EMBO J       Date:  1990-04       Impact factor: 11.598

4.  Subregions of a conserved part of the HIV gp41 transmembrane protein are differentially recognized by antibodies of infected individuals.

Authors:  U Certa; W Bannwarth; D Stüber; R Gentz; M Lanzer; S Le Grice; F Guillot; I Wendler; G Hunsmann; H Bujard
Journal:  EMBO J       Date:  1986-11       Impact factor: 11.598
  4 in total

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