Literature DB >> 3778482

The 14 alpha-demethylation of obtusifoliol by a cytochrome P-450 monooxygenase from higher plants' microsomes.

A Rahier, M Taton.   

Abstract

Microsomes isolated from corn embryos (Zea mays) can demethylate the 14 alpha-methyl group of obtusifoliol 2. An enzymatic assay has been developed for obtusifoliol 14 alpha-methyl-demethylase in higher plants. The enzymatic reaction was shown to occur sequentially, converting obtusifoliol 2 to 4 alpha-methyl-5 alpha-ergosta-8,24(28)-dien-3 beta-ol 4 via the trienol 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol 3 which was thoroughly identified. This enzymatic reaction is dependent of NADPH and molecular oxygen. It is inhibited by CO, menadione and specific inhibitors of cytochrome P-450, the CO inhibition being partially reversed by light. It is concluded that in Zea mays microsomes, obtusifoliol is demethylated at C-14 by a cytochrome P-450 containing monooxygenase system.

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Year:  1986        PMID： 3778482     DOI： 10.1016/0006-291x(86)90743-6

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  11 in total

1.  Increased sterol biosynthesis in tobacco calli resistant to a triazole herbicide which inhibits demethylation of 14α-methyl sterols.

Authors:  H Schaller; P Maillot-Vernier; G Belliard; P Benveniste
Journal:  Planta       Date:  1992-06       Impact factor: 4.116

2.  Immunochemical characterization of NADPH-cytochrome P-450 reductase from Jerusalem artichoke and other higher plants.

Authors:  I Benveniste; A Lesot; M P Hasenfratz; F Durst
Journal:  Biochem J       Date:  1989-05-01       Impact factor: 3.857

3.  In Vitro Selection of Calli Resistant to a Triazole Cytochrome-P-450-Obtusifoliol-14-Demethylase Inhibitor from Protoplasts of Nicotiana tabacum L. cv Xanthi.

Authors:  P Maillot-Vernier; H Schaller; P Benveniste; G Belliard
Journal:  Plant Physiol       Date:  1990-07       Impact factor: 8.340

Review 4.  Plant P450s as versatile drivers for evolution of species-specific chemical diversity.

Authors:  Björn Hamberger; Søren Bak
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2013-01-06       Impact factor: 6.237

5.  Oxygen radical generation by isolated microsomes from soybean seedlings.

Authors:  M Simontacchi; S Puntarulo
Journal:  Plant Physiol       Date:  1992-11       Impact factor: 8.340

6.  Cytochrome P-450-dependent 14 alpha-demethylation of lanosterol in Candida albicans.

Authors:  C A Hitchcock; S B Brown; E G Evans; D J Adams
Journal:  Biochem J       Date:  1989-06-01       Impact factor: 3.857

7.  Purification, characterization, and cDNA cloning of an NADPH-cytochrome P450 reductase from mung bean.

Authors:  M S Shet; K Sathasivan; M A Arlotto; M C Mehdy; R W Estabrook
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205

8.  Purification and partial characterization of NADPH-cytochrome c reductase from Petunia hybrida flowers.

Authors:  J G Menting; E Cornish; R K Scopes
Journal:  Plant Physiol       Date:  1994-10       Impact factor: 8.340

9.  Properties and structural requirements for substrate specificity of cytochrome P-450-dependent obtusifoliol 14 alpha-demethylase from maize (Zea mays) seedlings.

Authors:  M Taton; A Rahier
Journal:  Biochem J       Date:  1991-07-15       Impact factor: 3.857

10.  Xenobiotics: Substrates and inhibitors of the plant cytochrome P450.

Authors:  M Schalk; M A Pierrel; A Zimmerlin; Y Batard; F Durst; D Werck-Reichhart
Journal:  Environ Sci Pollut Res Int       Date:  1997       Impact factor: 4.223
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