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Literature DB >> 3777920

Immunoaffinity purification of subcellular particles and organelles.

Abstract

The application of immunoaffinity techniques to subcellular fractionation is reviewed and the basic principles underlying the various methods that have been successfully employed, identified. The requirement for organelle-specific antigens and high-avidity antibodies is discussed, as is the widespread use of indirect immunoadsorbents. Approaches for the optimization of immunoaffinity-based subcellular fractionation are suggested.

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Year: 1986 PMID： 3777920 DOI： 10.1007/bf02798907

Source DB: PubMed Journal: Appl Biochem Biotechnol ISSN： 0273-2289 Impact factor: 2.926

35 in total

1. Exploring cells with a centrifuge.

Authors: C Duve
Journal: Science Date: 1975-07-18 Impact factor: 47.728

2. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.

Authors: P L Ey; S J Prowse; C R Jenkin
Journal: Immunochemistry Date: 1978-07

3. Lymphoid cell fractionation on magnetic polyacrylamide--agarose beads.

Authors: J C Antoine; T Ternynck; M Rodrigot; S Avrameas
Journal: Immunochemistry Date: 1978-07

4. Neural cell sequestration on immunoaffinity columns.

Authors: A M Au; S Varon
Journal: Exp Cell Res Date: 1979-05 Impact factor: 3.905

5. The subpopulation of brain coated vesicles that carries synaptic vesicle proteins contains two unique polypeptides.

Authors: S R Pfeffer; R B Kelly
Journal: Cell Date: 1985-04 Impact factor: 41.582

6. Efficient isolation of genes by using antibody probes.

Authors: R A Young; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1983-03 Impact factor: 11.205

7. Labeled antibodies and their use in the immunoradiometric assay.

Authors: C N Hales; J S Woodhead
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

8. Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.

Authors: K K Stanley; J P Luzio
Journal: EMBO J Date: 1984-06 Impact factor: 11.598

9. Partial purification of presynaptic plasma membrane by immunoadsorption.

Authors: G P Miljanich; A R Brasier; R B Kelly
Journal: J Cell Biol Date: 1982-07 Impact factor: 10.539

10. Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue.

Authors: W D Matthew; L Tsavaler; L F Reichardt
Journal: J Cell Biol Date: 1981-10 Impact factor: 10.539

2 in total

1. The preparation of monoclonal antibodies to human bone and liver alkaline phosphatase and their use in immunoaffinity purification and in studying these enzymes when present in serum.

Authors: E M Bailyes; R N Seabrook; J Calvin; G A Maguire; C P Price; K Siddle; J P Luzio
Journal: Biochem J Date: 1987-06-15 Impact factor: 3.857

2. Distinct transport vesicles mediate the delivery of plasma membrane proteins to the apical and basolateral domains of MDCK cells.

Authors: A Wandinger-Ness; M K Bennett; C Antony; K Simons
Journal: J Cell Biol Date: 1990-09 Impact factor: 10.539

2 in total