Literature DB >> 3776313

An inexpensive easily available replacement for foetal calf serum in media for the in vitro cultivation of Leishmania spp.

D A Evans.   

Abstract

A replacement for foetal calf serum (FCS), made from heat-treated adult serum, in culture media designed for the in vitro cultivation of leishmanial promastigotes is described. The growth of 12 World Health Organization recommended strains of Leishmania in media containing heat-treated adult serum was compared with that obtained in media containing FCS. Growth of the promastigotes in the media with the FCS replacement was as good as or slightly better than that with FCS.

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Year:  1986        PMID： 3776313     DOI： 10.1007/bf00925475

Source DB:  PubMed          Journal:  Z Parasitenkd        ISSN： 0044-3255


  9 in total

1.  New culture medium for maintenance of tsetse tissues and growth of trypanosomatids.

Authors:  I Cunningham
Journal:  J Protozool       Date:  1977-05

2.  The utilization of glucose and proline by culture forms of Trypanosoma brucei.

Authors:  D A Evans; R C Brown
Journal:  J Protozool       Date:  1972-11

3.  Identification of an infective stage of Leishmania promastigotes.

Authors:  D L Sacks; P V Perkins
Journal:  Science       Date:  1984-03-30       Impact factor: 47.728

4.  Diagnosis of cutaneous leishmaniasis by in vitro cultivation of saline aspirates in Schneider's Drosophila Medium.

Authors:  L Hendricks; N Wright
Journal:  Am J Trop Med Hyg       Date:  1979-11       Impact factor: 2.345

5.  Characteristics of so-called natural antibodies in various normal sera against culture forms of Leishmania.

Authors:  G A Schmunis; R Herman
Journal:  J Parasitol       Date:  1970-10       Impact factor: 1.276

6.  Observations on the in vitro cultivation of Leishmania braziliensis.

Authors:  B C Walton; J J Shaw; R Lainson
Journal:  J Parasitol       Date:  1977-12       Impact factor: 1.276

7.  A simple monophasic medium for axenic culture of hemoflagellates.

Authors:  R L Berens; R Brun; S M Krassner
Journal:  J Parasitol       Date:  1976-06       Impact factor: 1.276

8.  The isolation and isoenzyme characterization of Leishmania braziliensis subsp. from patients with cutaneous leishmaniasis acquired in Belize.

Authors:  D A Evans; S M Lanham; C I Baldwin; W Peters
Journal:  Trans R Soc Trop Med Hyg       Date:  1984       Impact factor: 2.184

9.  Mechanism of lethal effect of human serum upon Leishmania donovani.

Authors:  R D Pearson; R T Steigbigel
Journal:  J Immunol       Date:  1980-11       Impact factor: 5.422
  9 in total

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