Literature DB >> 377229

The binding of tyrosinyl-5'-AMP to tyrosyl-tRNA synthetase (E.coli).

F Grosse, G Krauss, R Kownatzki, G Maass.   

Abstract

The binding between tyrosyl-tRNA synthetase (E.coli) and the alkylanalogue of the aminoacyladenylate, tyrosinyl-5'-AMP, has been investigated by fluorescence titrations and rapid mixing experiments. Tyrosyl-tRNA synthetase has two equivalent and independent binding sites for tyrosinyl-5'-AMP. The intrinsic binding constant is 4 x 10(7)M-1. The binding sites for tRNATyr and tyrosinyl-5'-AMP are independent of each other, the anticooperative mode of tRNA binding being preserved in the presence of tyrosinyl-5-AMP.

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Year:  1979        PMID： 377229      PMCID： PMC327796          DOI： 10.1093/nar/6.4.1631

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Distinct steps in the specific binding of tRNA to aminoacyl-tRNA synthetase. Temperature-jump studies on the serine-specific system from yeast and the tyrosine-specific system from Escherichia coli.

Authors:  D Riesner; A Pingoud; D Boehme; F Peters; G Maass
Journal:  Eur J Biochem       Date:  1976-09

2.  Ligand binding and enzymic catalysis coupled through subunits in tyrosyl-tRNA synthetase.

Authors:  A R Fersht; R S Mulvey; G L Koch
Journal:  Biochemistry       Date:  1975-01-14       Impact factor: 3.162

3.  Ligand binding stoichiometries, subunit structure, and slow transitions in aminoacyl-tRNA synthetases.

Authors:  R S Mulvey; A R Fersht
Journal:  Biochemistry       Date:  1977-09-06       Impact factor: 3.162

4.  Mechanism of tRNA-synthetase recognition: role of terminal A.

Authors:  G Krauss; D Riesner; G Maass
Journal:  Nucleic Acids Res       Date:  1977-07       Impact factor: 16.971

5.  The mechanism of action of methionyl-tRNA synthetase from Escherichia coli. 1. Fluorescence studies on tRNAMet binding as a function of ligands, ions and pH.

Authors:  S Blanquet; M Iwatsubo; J P Waller
Journal:  Eur J Biochem       Date:  1973-07-02

6.  Isoleucyl transfer ribonucleic acid synthetase of Escherichia coli B. A rapid kinetic investigation of the L-isoleucine-activating reaction.

Authors:  E Holler; M Calvin
Journal:  Biochemistry       Date:  1972-09-26       Impact factor: 3.162

7.  Active site titration and aminoacyl adenylate binding stoichiometry of aminoacyl-tRNA synthetases.

Authors:  A R Fersht; J S Ashford; C J Bruton; R Jakes; G L Koch; B S Hartley
Journal:  Biochemistry       Date:  1975-01-14       Impact factor: 3.162

8.  Demonstration of two active sites on a monomeric aminoacyl-tRNA synthetase. Possible roles of negative cooperativity and half-of-the-sites reactivity in oligomeric enzymes.

Authors:  A R Fersht
Journal:  Biochemistry       Date:  1975-01-14       Impact factor: 3.162

9.  Binding of tyrosine, adenosine triphosphate and analogues to crystalline tyrosyl transfer RNA synthetase.

Authors:  C Monteilhet; D M Blow
Journal:  J Mol Biol       Date:  1978-07-15       Impact factor: 5.469

10.  The mechanism of reaction of methionyl-tRNA synthetase from Escherichia coli. Interaction of the enzyme with ligands of the amino-acid-activation reaction.

Authors:  S Blanquet; G Fayat; J P Waller; M Iwatsubo
Journal:  Eur J Biochem       Date:  1972-01-21
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