| Literature DB >> 3772016 |
Abstract
Added to mitochondrial suspension, pyronin G changes the intensity of its fluorescence depending on the membrane potential (energy state) of the mitochondria. The mechanism of this effect is studied and a dependence is obtained between the membrane potential and the fluorescence intensity. This permits quantitative determination of the membrane potential by the changes in the fluorescence of the suspension. A method is proposed for measuring the membrane potential of vesicles in the -120 to -220 mV interval.Entities:
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Year: 1986 PMID: 3772016 DOI: 10.1016/0165-022x(86)90005-9
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN: 0165-022X