Osmoregulatory effects of prolactin and growth hormone in embryonic chicks.

Intact White Leghorn chick embryos were treated daily (on Days 6-13) with bovine prolactin (PRL) or ovine growth hormone (GH) at doses of 4-10 micrograms/g embryo wet wt. A control group received an equal volume of avian saline. [Na+] and [Cl-] were determined in allantoic fluid samples taken on Days 10, 12, and 14, and in amniotic fluid and blood plasma on Day 14. Allantoic fluid, amniotic fluid, and plasma osmolarities, embryo wet weight, hematocrit, and allantoic fluid volume were also determined on Day 14. PRL-treated embryos showed significantly lower allantoic [Na+] and [Cl-] compared to controls at Days 10, 12, and 14. Allantoic fluid osmolarity was reduced, and plasma osmolarity increased, at Day 14 in PRL-treated embryos. By contrast, PRL had no effect on allantoic volume, amniotic fluid [Na+], [Cl-], or osmolarity, plasma [Na+] or [Cl-], hematocrit, or embryo wet weight. GH-treated embryos showed significantly reduced allantoic [Na+] at both Days 10 and 14, but no other treatment effect. Calculations show that the decrease in total allantoic Na+ seen in PRL-treated embryos is equivalent in magnitude to 10% of the total egg Na+. Results from studies on embryonic amphibians and mammals suggest that this sodium is likely to be sequestered in an expanded extracellular volume.