The subcellular distribution and levels of calmodulin in jejunal biopsies from control subjects and patients with coeliac disease.

A radioimmunoassay has been established and validated for the estimation of calmodulin levels in human peroral jejunal biopsy samples. Total levels were similar in biopsies from control subjects and patients with coeliac disease, both in relapse and remission. The subcellular distribution of calmodulin was determined in biopsy samples from control subjects and a patient with coeliac disease in relapse by rapid single-step analytical subcellular fractionation and immunoassay. In both situations most (greater than 70%) of the calmodulin was recovered in the cytosolic fractions with no selective enrichment in any single organelle.