Long-term effects of parallel fiber loss in the cerebellar cortex of the adult and weanling rat.

Short- and long-term effects of parallel fiber deafferentation of adult and weanling cerebellar cortex were investigated following parasagittal transections of the lateral cerebellar hemisphere. Short-term electron microscopic examination revealed that parallel fibers undergo rapid electron-dense degeneration within 5 days of axotomy. These axons were the only neuronal elements immediately affected by the lesion. The continued maintenance of Purkinje cell terminal branchlets and stellate cell dendrites is dependent upon the presence of an adequate parallel fiber milieu. Morphological evidence is provided which suggests that Purkinje cell dendritic spines may be phagocytically removed by Bergmann glial cells following parallel fiber loss. Although a marked decrease was reported in the number of spines projecting from terminal branchlets following deafferentation of both adult and weanling rats, these data suggest that some spines are capable of increasing their length. The elongation of these spines may represent a form of dendritic plasticity. No evidence was found to suggest that deafferentated terminal branchlets are receptive to forming heterologous synaptic contacts. The primary response to parallel fiber deafferentation for both the adult and weanling cerebellum therefore appears to be transneuronal degeneration.