Literature DB >> 3767285

A method for the identification of apolipoprotein E isoforms employing chemical precipitation and flat bed isoelectric focusing in agarose.

J W Kane, E Gowland.   

Abstract

A method is described for the identification of apolipoprotein E phenotypes. Lipoproteins were precipitated by magnesium chloride/phosphotungstate reagent and isoelectric focusing performed on flat-bed agarose. Sensitivity was enhanced by blotting with nitrocellulose prior to staining. The system described requires no ultracentrifugation step and allows up to 20 samples to be processed in each run. Agarose has some advantages over the more commonly used polyacrylamide gels for isoelectric focusing.

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Year:  1986        PMID： 3767285     DOI： 10.1177/000456328602300504

Source DB:  PubMed          Journal:  Ann Clin Biochem        ISSN： 0004-5632            Impact factor:   2.057


  5 in total

1.  Both total and phosphorylated tau are increased in Alzheimer's disease.

Authors:  M Sjögren; P Davidsson; M Tullberg; L Minthon; A Wallin; C Wikkelso; A K Granérus; H Vanderstichele; E Vanmechelen; K Blennow
Journal:  J Neurol Neurosurg Psychiatry       Date:  2001-05       Impact factor: 10.154

2.  A population study of apoE genotype at the age of 85: relation to dementia, cerebrovascular disease, and mortality.

Authors:  I Skoog; C Hesse; O Aevarsson; S Landahl; J Wahlström; P Fredman; K Blennow
Journal:  J Neurol Neurosurg Psychiatry       Date:  1998-01       Impact factor: 10.154

3.  Cerebrospinal fluid tau protein as a biochemical marker for Alzheimer's disease: a community based follow up study.

Authors:  N Andreasen; E Vanmechelen; A Van de Voorde; P Davidsson; C Hesse; S Tarvonen; I Räihä; L Sourander; B Winblad; K Blennow
Journal:  J Neurol Neurosurg Psychiatry       Date:  1998-03       Impact factor: 10.154

4.  LNA-enhanced detection of single nucleotide polymorphisms in the apolipoprotein E.

Authors:  Nana Jacobsen; Joan Bentzen; Michael Meldgaard; Mogens Havsteen Jakobsen; Mogens Fenger; Sakari Kauppinen; Jan Skouv
Journal:  Nucleic Acids Res       Date:  2002-10-01       Impact factor: 16.971

5.  Omega-3 Ethyl Ester Concentrate Decreases Total Apolipoprotein CIII and Increases Antithrombin III in Postmyocardial Infarction Patients.

Authors:  E Swahn; H von Schenck; A G Olsson
Journal:  Clin Drug Investig       Date:  1998       Impact factor: 2.859
  5 in total

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