The failure of flurofamide to control ureaplasma in bovine semen.

The flurofamide sensitivities of 21 bovine ureaplasma isolates were determined using the metabolic inhibition method. The 21 isolates included seven each of vaginal, preputial and seminal origin. The minimum inhibitory concentrations of flurofamide ranged from 0.0125 to 0.2 meg/mL against a ureaplasma titer of 10(4) organisms/mL. The minimum lethal concentrations ranged from less than or equal to 0.1 to 3.2 mcg/mL. Flurofamide was then evaluated in a system comparable to the procedure for semen extension with respect to temperature, time and dilution. The compound was found to be ineffective in reducing ureaplasma numbers in this system at levels up to 1500 mcg/mL.