Literature DB >> 3755692

Isolation of an alpha 1 type-IV collagen cDNA clone using a synthetic oligodeoxynucleotide.

P Nath, M Laurent, E Horn, M E Sobel, G Zon, G Vogeli.   

Abstract

We have isolated a cDNA clone (pCIV-1-225) for the alpha 1 subunit of basement membrane (type IV) collagen from a cDNA library made from Engelbreth-Holm-Swarm mouse tumor RNA. The cDNA library was screened with synthetic oligodeoxynucleotides derived from published amino acid (aa) sequences (Schuppan et al., 1982). Nucleotide sequence data established the identity of our cDNA clone to encode an alpha 1 type IV collagen. This clone contains 270 aa of the helical region and has three interruptions in the Gly-X-Y repeat unit.

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Year:  1986        PMID: 3755692     DOI: 10.1016/0378-1119(86)90220-9

Source DB:  PubMed          Journal:  Gene        ISSN: 0378-1119            Impact factor:   3.688


  6 in total

1.  Evolution of collagen IV genes from a 54-base pair exon: a role for introns in gene evolution.

Authors:  G Butticè; P Kaytes; J D'Armiento; G Vogeli; M Kurkinen
Journal:  J Mol Evol       Date:  1990-06       Impact factor: 2.395

2.  Gastric ulcer healing in the rat: kinetics and localisation of de novo procollagen synthesis.

Authors:  M Shahin; A Gillessen; T Pohle; C Weber; D Schuppan; H Herbst; W Domschke
Journal:  Gut       Date:  1997-08       Impact factor: 23.059

3.  Regulatory role of eicosanoids in extracellular matrix overproduction induced by long-term exposure to high glucose in cultured rat mesangial cells.

Authors:  F Pricci; G Pugliese; P Menè; G Romeo; G Romano; G Galli; A Casini; C M Rotella; U DiMario; F Pugliese
Journal:  Diabetologia       Date:  1996-09       Impact factor: 10.122

4.  Increased activity of the insulin-like growth factor system in mesangial cells cultured in high glucose conditions. Relation to glucose-enhanced extracellular matrix production.

Authors:  G Pugliese; F Pricci; N Locuratolo; G Romeo; G Romano; S Giannini; B Cresci; G Galli; C M Rotella; U Di Mario
Journal:  Diabetologia       Date:  1996-07       Impact factor: 10.122

5.  Epidermal cancer associated with expression of human papillomavirus type 16 E6 and E7 oncogenes in the skin of transgenic mice.

Authors:  P F Lambert; H Pan; H C Pitot; A Liem; M Jackson; A E Griep
Journal:  Proc Natl Acad Sci U S A       Date:  1993-06-15       Impact factor: 11.205

6.  Selective immunoreactivities of kidney basement membranes to monoclonal antibodies against laminin: localization of the end of the long arm and the short arms to discrete microdomains.

Authors:  D R Abrahamson; M H Irwin; P L St John; E W Perry; M A Accavitti; L W Heck; J R Couchman
Journal:  J Cell Biol       Date:  1989-12       Impact factor: 10.539

  6 in total

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