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Literature DB >> 3754926

In situ cDNA:mRNA hybridization: development of a technique to measure mRNA levels in individual cells.

Abstract

In this article we have described our protocol for in situ cDNA:mRNA hybridization and a variety of methodological considerations we have entertained to optimize the procedure. It should be stressed that each different system will have its own special characteristics and require optimization to obtain maximal and reproducible signals. We believe that by following a methodological logic as outlined here, researchers should be able to establish the in situ cDNA:mRNA hybridization protocol with their probes and tissue systems.

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Year: 1986 PMID： 3754926 DOI： 10.1016/0076-6879(86)24037-9

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

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Cited

13 in total

1. Analysis of the role of microsomal triglyceride transfer protein in the liver of tissue-specific knockout mice.

Authors: M Raabe; M M Véniant; M A Sullivan; C H Zlot; J Björkegren; L B Nielsen; J S Wong; R L Hamilton; S G Young
Journal: J Clin Invest Date: 1999-05 Impact factor: 14.808

2. Multiple regulatory domains in the mouse mammary tumor virus long terminal repeat revealed by analysis of fusion genes in transgenic mice.

Authors: T A Stewart; P G Hollingshead; S L Pitts
Journal: Mol Cell Biol Date: 1988-01 Impact factor: 4.272

3. The effects of estrogens on tumor growth and on prolactin and growth hormone mRNA expression in rat pituitary tissues.

Authors: R V Lloyd; M Cano; T D Landefeld
Journal: Am J Pathol Date: 1988-11 Impact factor: 4.307

Review 4. Hybridization histochemistry.

Authors: J D Penschow; J Haralambidis; P E Darling; I A Darby; E M Wintour; G W Tregear; J P Coghlan
Journal: Experientia Date: 1987-07-15

In situ cDNA:mRNA hybridization: development of a technique to measure mRNA levels in individual cells.

1. Analysis of the role of microsomal triglyceride transfer protein in the liver of tissue-specific knockout mice.

2. Multiple regulatory domains in the mouse mammary tumor virus long terminal repeat revealed by analysis of fusion genes in transgenic mice.

3. The effects of estrogens on tumor growth and on prolactin and growth hormone mRNA expression in rat pituitary tissues.

Review 4. Hybridization histochemistry.

5. Gene expression of gonadotropin-releasing hormone in early pregnant rat and steroid hormone exposed mouse uteri.

6. Murine tissue factor gene expression in vivo. Tissue and cell specificity and regulation by lipopolysaccharide.

7. Effects of energy intake on type 1 plasminogen activator inhibitor levels in glomeruli of lupus-prone B/W mice.

8. Natriuretic peptide receptor mRNAs in the rat and human heart.

9. Developmental expression of transforming growth factors alpha and beta in mouse fetus.

10. Cellular localization of type 1 plasminogen activator inhibitor messenger RNA and protein in murine renal tissue.