Literature DB >> 3754856

A method for the fine-structural demonstration of lectin receptors.

K Ichev, W Ovtscharoff, H Franz, J Mohr.   

Abstract

A new two-step method using an Fc-fragment/ferritin conjugate as a marker for the visualization of lectin-binding sites on neuronal and other cell membranes is described. In this study of rat synaptosomes, three lectins were tested: concanavalin A, mistletoe lectin I and wheat germ agglutinin. The specificity of the method was proved by control experiments.

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Year:  1986        PMID： 3754856     DOI： 10.1007/bf00499831

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  6 in total

1.  Reaction of lectins with human erythrocytes. II. Mapping of conA receptors by freeze-etching electron microscopy.

Authors:  T Bachi; H P Schnebli
Journal:  Exp Cell Res       Date:  1975-03-15       Impact factor: 3.905

2.  The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation.

Authors:  E G GRAY; V P WHITTAKER
Journal:  J Anat       Date:  1962-01       Impact factor: 2.610

3.  Mistletoe lectin I binding sites on the synaptosomes of the rat cerebral cortex.

Authors:  K Ichev; W Ovtscharoff; P Bergmann; H Franz; L Venkov
Journal:  Acta Histochem       Date:  1985       Impact factor: 2.479

4.  Binding of Concanavalin A to the envelope of two murine RNA tumour viruses.

Authors:  J Calafat; P C Hageman
Journal:  J Gen Virol       Date:  1972-01       Impact factor: 3.891

Review 5.  Cytochemistry of cell glycoconjugates.

Authors:  J Schrével; D Gros; M Monsigny
Journal:  Prog Histochem Cytochem       Date:  1981

6.  Combination of immunological and lectin reactions in affinity histochemistry: proposition of the term affinitin.

Authors:  H Franz; P Bergmann; P Ziska
Journal:  Histochemistry       Date:  1979-02-21
  6 in total

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