Literature DB >> 3753871

Selective labelling of Cys-10 on actin.

J A Barden, M Miki, C G dos Remedios.   

Abstract

Conditions are described for the selective modification of Cys-10 on actin achieved following the blocking of the more reactive Cys-374. Labelling of Cys-10 did not affect the formation of actin filaments. This residue should be capable of serving as a site for fluorescence donors or acceptors and thus will be a useful locus to probe F-actin structure.

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Year:  1986        PMID: 3753871

Source DB:  PubMed          Journal:  Biochem Int        ISSN: 0158-5231


  3 in total

Review 1.  Structure of actin observed by fluorescence resonance energy transfer spectroscopy.

Authors:  M Miki; S I O'Donoghue; C G Dos Remedios
Journal:  J Muscle Res Cell Motil       Date:  1992-04       Impact factor: 2.698

Review 2.  Fluorescence resonance energy transfer measurements of distances in actin and myosin. A critical evaluation.

Authors:  C G dos Remedios; M Miki; J A Barden
Journal:  J Muscle Res Cell Motil       Date:  1987-04       Impact factor: 2.698

3.  Actin dynamics studied by solid-state NMR spectroscopy.

Authors:  L Phillips; F Separovic; B A Cornell; J A Barden; C G dos Remedios
Journal:  Eur Biophys J       Date:  1991       Impact factor: 1.733

  3 in total

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