Literature DB >> 3753720

Characterization of proopiomelanocortin mRNA detected by in situ hybridization.

J E Kelsey, S J Watson, S Burke, H Akil, J L Roberts.   

Abstract

In situ hybridization is a method for detecting specific nucleotide sequences by using a labeled complementary nucleic acid probe. We have characterized in situ hybridization in rat pituitary using a cDNA probe directed against a portion of proopiomelanocortin (POMC) mRNA to show its specificity in tissue sections. This characterization includes the size of the target mRNA from tissue processed for in situ hybridization, the dissociation pattern of the cDNA:mRNA hybrid, and quantitation of changes in POMC mRNA levels in the rat pituitary intermediate lobe detected both by in situ hybridization and "dot blot" techniques.

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Year:  1986        PMID： 3753720      PMCID： PMC6568624     

Source DB:  PubMed          Journal:  J Neurosci        ISSN： 0270-6474            Impact factor:   6.167


  8 in total

Review 1.  Neuropeptide gene expression and neural activity: assessing a working hypothesis in nucleus caudalis and dorsal horn neurons expressing preproenkephalin and preprodynorphin.

Authors:  G R Uhl; T Nishimori
Journal:  Cell Mol Neurobiol       Date:  1990-03       Impact factor: 5.046

2.  The effects of estrogens on tumor growth and on prolactin and growth hormone mRNA expression in rat pituitary tissues.

Authors:  R V Lloyd; M Cano; T D Landefeld
Journal:  Am J Pathol       Date:  1988-11       Impact factor: 4.307

3.  Human growth hormone and prolactin secreting pituitary adenomas analyzed by in situ hybridization.

Authors:  R V Lloyd; M Cano; W F Chandler; A L Barkan; E Horvath; K Kovacs
Journal:  Am J Pathol       Date:  1989-03       Impact factor: 4.307

4.  Thyroid hormones regulate levels of thyrotropin-releasing-hormone mRNA in the paraventricular nucleus.

Authors:  K J Koller; R S Wolff; M K Warden; R T Zoeller
Journal:  Proc Natl Acad Sci U S A       Date:  1987-10       Impact factor: 11.205

5.  Analysis of endocrine active and clinically silent corticotropic adenomas by in situ hybridization.

Authors:  R V Lloyd; K Fields; L Jin; E Horvath; K Kovacs
Journal:  Am J Pathol       Date:  1990-08       Impact factor: 4.307

6.  In situ hybridization study of pro-opiomelanocortin (POMC) gene expression in human pituitary corticotrophs and their adenomas.

Authors:  L Stefaneanu; K Kovacs; E Horvath; R V Lloyd
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1991

7.  Detection of proopiomelanocortin mRNA by in situ hybridization with an oligonucleotide probe.

Authors:  M E Lewis; T G Sherman; S Burke; H Akil; L G Davis; R Arentzen; S J Watson
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205

8.  Fecal dysbiosis in infants with cystic fibrosis is associated with early linear growth failure.

Authors:  Hillary S Hayden; Alexander Eng; Christopher E Pope; Mitchell J Brittnacher; Anh T Vo; Eli J Weiss; Kyle R Hager; Bryan D Martin; Daniel H Leung; Sonya L Heltshe; Elhanan Borenstein; Samuel I Miller; Lucas R Hoffman
Journal:  Nat Med       Date:  2020-01-20       Impact factor: 53.440
  8 in total

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