Literature DB >> 3753440

Purification of cathepsin B by a new form of affinity chromatography.

D H Rich, M A Brown, A J Barrett.   

Abstract

Human cathepsin B was purified by affinity chromatography on the semicarbazone of Gly-Phe-glycinal linked to Sepharose 4B, with elution by 2,2'-dipyridyl disulphide at pH 4.0. The product obtained in high yield by the single step from crude starting material was 80-100% active cathepsin B. The possibility that this new form of affinity chromatography may be of general usefulness in the purification of cysteine proteinases is discussed.

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Year:  1986        PMID： 3753440      PMCID： PMC1146748          DOI： 10.1042/bj2350731

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  Interaction of papain with derivatives of phenylalanylglycinal.

Authors:  J A Mattis; J B Henes; J S Fruton
Journal:  J Biol Chem       Date:  1977-10-10       Impact factor: 5.157

2.  An agarose mercurial column for the separation of mercaptopapain and nonmercaptopapain.

Authors:  L A Sluyterman; J Wijdenes
Journal:  Biochim Biophys Acta       Date:  1970-03-31

Review 3.  Cathepsin B, Cathepsin H, and cathepsin L.

Authors:  A J Barrett; H Kirschke
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

4.  Human cathepsin H.

Authors:  W N Schwartz; A J Barrett
Journal:  Biochem J       Date:  1980-11-01       Impact factor: 3.857

5.  Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols.

Authors:  K Brocklehurst; G Little
Journal:  Biochem J       Date:  1973-05       Impact factor: 3.857

6.  Inactivation of cathepsin B by active site-directed disulfide exchange. Application in covalent affinity chromatography.

Authors:  B Evans; E Shaw
Journal:  J Biol Chem       Date:  1983-09-10       Impact factor: 5.157

7.  Preparation of cathepsins B and H by covalent chromatography and characterization of their catalytic sites by reaction with a thiol-specific two-protonic-state reactivity probe. Kinetic study of cathepsins B and H extending into alkaline media and a rapid spectroscopic titration of cathepsin H at pH 3-4.

Authors:  F Willenbrock; K Brocklehurst
Journal:  Biochem J       Date:  1985-04-15       Impact factor: 3.857

8.  Transition-state affinity chromatography of trypsin-like proteinases with dipeptidyl argininal ligands.

Authors:  A H Patel; A Ahsan; B P Suthar; R M Schultz
Journal:  Biochim Biophys Acta       Date:  1983-10-28

9.  Human cathepsin B1. Purification and some properties of the enzyme.

Authors:  A J Barrett
Journal:  Biochem J       Date:  1973-04       Impact factor: 3.857

10.  Differences in the interaction of the catalytic groups of the active centres of actinidin and papain. Rapid purification of fully active actinidin by covalent chromatography and characterization of its active centre by use of two-protonic-state reactivity probes.

Authors:  K Brocklehurst; B S Baines; J P Malthouse
Journal:  Biochem J       Date:  1981-09-01       Impact factor: 3.857
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  24 in total

1.  Proteolytic processing and glycosylation of cathepsin B. The role of the primary structure of the latent precursor and of the carbohydrate moiety for cell-type-specific molecular forms of the enzyme.

Authors:  L Mach; K Stüwe; A Hagen; C Ballaun; J Glössl
Journal:  Biochem J       Date:  1992-03-01       Impact factor: 3.857

2.  A model to explain the pH-dependent specificity of cathepsin B-catalysed hydrolyses.

Authors:  H E Khouri; C Plouffe; S Hasnain; T Hirama; A C Storer; R Ménard
Journal:  Biochem J       Date:  1991-05-01       Impact factor: 3.857

3.  Design, synthesis, and evaluation of inhibitors of cathepsin L: Exploiting a unique thiocarbazate chemotype.

Authors:  Michael C Myers; Parag P Shah; Mary Pat Beavers; Andrew D Napper; Scott L Diamond; Amos B Smith; Donna M Huryn
Journal:  Bioorg Med Chem Lett       Date:  2008-05-01       Impact factor: 2.823

4.  Cartilage proteoglycan aggregate is degraded more extensively by cathepsin L than by cathepsin B.

Authors:  Q Nguyen; J S Mort; P J Roughley
Journal:  Biochem J       Date:  1990-03-01       Impact factor: 3.857

5.  Expression of functional recombinant human procathepsin B in mammalian cells.

Authors:  W P Ren; R Fridman; J R Zabrecky; L D Morris; N A Day; B F Sloane
Journal:  Biochem J       Date:  1996-11-01       Impact factor: 3.857

6.  Degradation of laminin by human tumor cathepsin B.

Authors:  T T Lah; M R Buck; K V Honn; J D Crissman; N C Rao; L A Liotta; B F Sloane
Journal:  Clin Exp Metastasis       Date:  1989 Jul-Aug       Impact factor: 5.150

7.  A comparison of four cathepsins (B, L, N and S) with collagenolytic activity from rabbit spleen.

Authors:  R A Maciewicz; D J Etherington
Journal:  Biochem J       Date:  1988-12-01       Impact factor: 3.857

8.  Degradation of extracellular-matrix proteins by human cathepsin B from normal and tumour tissues.

Authors:  M R Buck; D G Karustis; N A Day; K V Honn; B F Sloane
Journal:  Biochem J       Date:  1992-02-15       Impact factor: 3.857

9.  Increased body temperature accelerates aggregation of the Leu-68-->Gln mutant cystatin C, the amyloid-forming protein in hereditary cystatin C amyloid angiopathy.

Authors:  M Abrahamson; A Grubb
Journal:  Proc Natl Acad Sci U S A       Date:  1994-02-15       Impact factor: 11.205

10.  Activation of procathepsin B in human hepatoma cells: the conversion into the mature enzyme relies on the action of cathepsin B itself.

Authors:  L Mach; H Schwihla; K Stüwe; A D Rowan; J S Mort; J Glössl
Journal:  Biochem J       Date:  1993-07-15       Impact factor: 3.857
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