| Literature DB >> 3745116 |
Abstract
Rhizobium leguminosarum cells were separated into four distinct fractions by using density gradient centrifugation for the separation of the outer and cytoplasmic membranes and lysozyme-EDTA treatment of whole cells for the isolation of the periplasmic and cytoplasmic fractions. These methods allowed the subcellular localization of R. leguminosarum proteins.Entities:
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Year: 1986 PMID: 3745116 PMCID: PMC215987 DOI: 10.1128/jb.167.3.1083-1085.1986
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490